Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bernhart, E; Kollroser, M; Rechberger, G; Reicher, H; Heinemann, A; Schratl, P; Hallström, S; Wintersperger, A; Nusshold, C; DeVaney, T; Zorn-Pauly, K; Malli, R; Graier, W; Malle, E; Sattler, W.
Lysophosphatidic acid receptor activation affects the C13NJ microglia cell line proteome leading to alterations in glycolysis, motility, and cytoskeletal architecture.
Proteomics. 2010; 10(1):141-158
Doi: 10.1002/pmic.200900195
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- Führende Autor*innen der Med Uni Graz
- Bernhart Eva Maria
- Sattler Wolfgang
- Co-Autor*innen der Med Uni Graz
- DeVaney Trevor
- Graier Wolfgang
- Hallström Seth
- Heinemann Akos
- Hinteregger Helga
- Kollroser Manfred
- Luschnig Petra
- Malle Ernst
- Malli Roland
- Nusshold Christoph
- Wintersperger Andrea
- Zorn-Pauly Klaus
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- Abstract:
- Microglia, the immunocompetent cells of the CNS, are rapidly activated in response to injury and microglia migration towards and homing at damaged tissue plays a key role in CNS regeneration. Lysophosphatidic acid (LPA) is involved in signaling events evoking microglia responses through cognate G protein-coupled receptors. Here we show that human immortalized C13NJ microglia express LPA receptor subtypes LPA(1), LPA(2), and LPA(3) on mRNA and protein level. LPA activation of C13NJ cells induced Rho and extracellular signal-regulated kinase activation and enhanced cellular ATP production. In addition, LPA induced process retraction, cell spreading, led to pronounced changes of the actin cytoskeleton and reduced cell motility, which could be reversed by inhibition of Rho activity. To get an indication about LPA-induced global alterations in protein expression patterns a 2-D DIGE/LC-ESI-MS proteomic approach was applied. On the proteome level the most prominent changes in response to LPA were observed for glycolytic enzymes and proteins regulating cell motility and/or cytoskeletal dynamics. The present findings suggest that naturally occurring LPA is a potent regulator of microglia biology. This might be of particular relevance in the pathophysiological context of neurodegenerative disorders where LPA concentrations can be significantly elevated in the CNS.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line -
- Cell Movement -
- Cytoskeleton - metabolism
- Gene Expression Regulation -
- Glycolysis -
- Humans -
- Lysophospholipids - metabolism
- Microglia - cytology
- Microglia - metabolism
- Mitogen-Activated Protein Kinase 1 - metabolism
- Mitogen-Activated Protein Kinase 3 - metabolism
- Proteome - metabolism
- Receptors, Lysophosphatidic Acid - genetics
- Receptors, Lysophosphatidic Acid - metabolism
- Signal Transduction -
- rho GTP-Binding Proteins - metabolism
- Find related publications in this database (Keywords)
- 2-D DIGE
- Cell biology
- Lipids
- Migration
- MS
- Rho