Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Bernhart, E; Kollroser, M; Rechberger, G; Reicher, H; Heinemann, A; Schratl, P; Hallström, S; Wintersperger, A; Nusshold, C; DeVaney, T; Zorn-Pauly, K; Malli, R; Graier, W; Malle, E; Sattler, W.
Lysophosphatidic acid receptor activation affects the C13NJ microglia cell line proteome leading to alterations in glycolysis, motility, and cytoskeletal architecture.
Proteomics. 2010; 10(1):141-158 Doi: 10.1002/pmic.200900195 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG Google Scholar


Führende Autor*innen der Med Uni Graz
Bernhart Eva Maria
Sattler Wolfgang
Co-Autor*innen der Med Uni Graz
DeVaney Trevor
Graier Wolfgang
Hallström Seth
Heinemann Akos
Hinteregger Helga
Kollroser Manfred
Luschnig Petra
Malle Ernst
Malli Roland
Nusshold Christoph
Wintersperger Andrea
Zorn-Pauly Klaus

Dimensions Citations:

Plum Analytics:

Scite (citation analytics):

Microglia, the immunocompetent cells of the CNS, are rapidly activated in response to injury and microglia migration towards and homing at damaged tissue plays a key role in CNS regeneration. Lysophosphatidic acid (LPA) is involved in signaling events evoking microglia responses through cognate G protein-coupled receptors. Here we show that human immortalized C13NJ microglia express LPA receptor subtypes LPA(1), LPA(2), and LPA(3) on mRNA and protein level. LPA activation of C13NJ cells induced Rho and extracellular signal-regulated kinase activation and enhanced cellular ATP production. In addition, LPA induced process retraction, cell spreading, led to pronounced changes of the actin cytoskeleton and reduced cell motility, which could be reversed by inhibition of Rho activity. To get an indication about LPA-induced global alterations in protein expression patterns a 2-D DIGE/LC-ESI-MS proteomic approach was applied. On the proteome level the most prominent changes in response to LPA were observed for glycolytic enzymes and proteins regulating cell motility and/or cytoskeletal dynamics. The present findings suggest that naturally occurring LPA is a potent regulator of microglia biology. This might be of particular relevance in the pathophysiological context of neurodegenerative disorders where LPA concentrations can be significantly elevated in the CNS.
Find related publications in this database (using NLM MeSH Indexing)
Cell Line -
Cell Movement -
Cytoskeleton - metabolism
Gene Expression Regulation -
Glycolysis -
Humans -
Lysophospholipids - metabolism
Microglia - cytology
Microglia - metabolism
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3 - metabolism
Proteome - metabolism
Receptors, Lysophosphatidic Acid - genetics
Receptors, Lysophosphatidic Acid - metabolism
Signal Transduction -
rho GTP-Binding Proteins - metabolism

Find related publications in this database (Keywords)
Cell biology
© Med Uni Graz Impressum