Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Reininger, R; Varga, EM; Zach, M; Balic, N; Lindemeier, AD; Swoboda, I; Grönlund, H; van Hage, M; Rumpold, H; Valenta, R; Spitzauer, S.
Detection of an allergen in dog dander that cross-reacts with the major cat allergen, Fel d 1.
Clin Exp Allergy. 2007; 37(1): 116-124.
Doi: 10.1111/j.1365-2222.2006.02611.x
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Varga Eva-Maria
- Zach Maximilian
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- Abstract:
- BACKGROUND: A considerable proportion of animal-allergic patients are sensitized to both cat and dog allergens but knowledge about cross-reactive allergens in cat and dog dander is limited. OBJECTIVE: To investigate whether dog dander contains an allergen that cross-reacts with the major cat allergen, Fel d 1. METHODS: Recombinant Fel d 1 with the same immunological properties as natural Fel d 1 was used for quantitative (CAP) IgE competition experiments performed with sera obtained from cat-allergic patients (n=36). A Fel d 1 cross-reactive dog allergen was characterized by one- and two-dimensional immunoblotting using rFel d 1 for IgE inhibition experiments and with monospecific, polyclonal rabbit anti-recombinant Fel d 1 antibodies. RESULTS: In 25% of Fel d 1-reactive cat-allergic patients, more than 50% inhibition of IgE reactivity to dog allergens was achieved with recombinant Fel d 1. An Fel d 1 cross-reactive 20 kDa allergen with a pI of approximately 3.4 was detected in dander extracts of several different dog breeds. CONCLUSION: This is the first report demonstrating the presence of an Fel d 1-like allergen in dog dander extracts, which may be responsible for double positivity to cat and dog in serology. However, the clinical relevance of this cross-sensitization needs to be confirmed. These results are important for the diagnostic and therapeutic use of dog dander allergen extracts.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Allergens - immunology
- Animals - immunology
- Cats - immunology
- Child - immunology
- Child, Preschool - immunology
- Cross Reactions - immunology
- Dogs - immunology
- Dust - immunology
- Electrophoresis, Gel, Two-Dimensional - immunology
- Environmental Pollution - immunology
- Female - immunology
- Glycoproteins - immunology
- Humans - immunology
- Hypersensitivity - immunology
- Immune Sera - immunology
- Immunoblotting - immunology
- Immunoglobulin E - blood
- Male - blood
- Rabbits - blood
- Recombinant Proteins - immunology
- Serum Albumin - immunology
- Skin Tests - immunology
- Find related publications in this database (Keywords)
- cat allergy
- cross-reactivity
- dog allergy
- Fel d 1
- major cat allergen
- recombinant allergen