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Gugerell, A; Kober, J; Schmid, M; Nickl, S; Kamolz, LP; Keck, M.
Botulinum toxin A and lidocaine have an impact on adipose-derived stem cells, fibroblasts, and mature adipocytes in vitro.
J Plast Reconstr Aesthet Surg. 2014; 67(9):1276-1281
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Autor/innen der Med Uni Graz:
Kamolz Lars-Peter
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Abstract:
Lipofilling with autologous fat tissue is widely used in plastic and reconstructive surgery to treat soft-tissue deficiency. Unfortunately, implanted cells disappear gradually and make it difficult to predict the resorption rate. Several adjuvants are used to improve the success of fat tissue grafting. In this study, the effect of botulinum toxin (BoNT) on mature adipocytes, as well as adipose-derived stem cells (ASC) and fibroblasts was evaluated. As lidocaine is the most prevalent drug to anesthetize the donor site as well as the area to be treated with autologous fat, this local anesthetic was examined too. Primary ASCs, fibroblasts, and mature adipocytes were exposed to 1, 10, and 20 IU/ml BoNT A and 1% lidocaine. Cells were tested on proliferation, viability, and LDH release. Adipogenic differentiation potential was evaluated by quantitative real-time PCR analyzing the expression of FABP4. BoNT had no significant influence on the proliferation or viability of tested cells. By trend, low concentrations of BoNT improved adipogenic potential of ASCs. Lidocaine had a strong diminishing effect on the proliferation of ASCs and fibroblasts and on the viability of these cells. Mature adipocytes show no significant inferior viability after BoNT or lidocaine treatment. BoNT has no negative effect on ASCs, mature adipocytes, or fibroblasts in vitro. Lidocaine (1%) negatively influences the proliferation and viability of fibroblasts and partly of ASCs but not of mature adipocytes. Copyright © 2014 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Adipocytes - drug effects
Adipocytes - transplantation
Adipose Tissue - cytology
Anesthetics, Local - pharmacology
Botulinum Toxins, Type A - pharmacology
Cell Differentiation - drug effects
Cells, Cultured -
Fibroblasts - drug effects
Fibroblasts - transplantation
Humans -
In Vitro Techniques -
Lidocaine - pharmacology
Stem Cell Transplantation -
Stem Cells - drug effects
Transplant Donor Site - surgery

Find related publications in this database (Keywords)
Botulinum toxin A
Adipocytes
Adipose-derived stem cells
Fibroblasts
Lidocaine
Autologous fat grafting
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