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SHR Neuro Krebs Kardio Lipid

Santigli, E; Koller, M; Klug, B.
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children.
J Vis Exp. 2017; 8(130): [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Klug Barbara
Koller Martin
Santigli Elisabeth
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Abstract:
Oral biofilm and its molecular analysis provide a basis for investigating various dental research and clinical questions. Knowledge of biofilm composition leads to a better understanding of cariogenic and periopathogenic mechanisms. Microbial changes taking place in the oral cavity during childhood are of interest for several reasons. The evolution of the child oral microbiota and shifts in its composition need to be analyzed further to understand and possibly prevent the onset of disease. At the same time, advanced knowledge of the natural composition of oral biofilm is needed. Early stages of caries-free permanent dentition with healthy gums provide a widely unaffected subgingival habitat that can serve as an in situ baseline for studying features of oral health and disease. Analysis of children's oral biofilm during different stages in life is thus an important theme in the field. Modern molecular analysis methods can provide comprehensive information about the bacterial diversity of such biofilms. To enable microbiota data comparison, it is important to standardize each step in the procedure for molecular data generation. This procedure spans from clinical sampling, Next Generation Sequencing (NGS), bioinformatic data processing, to taxonomic interpretation. One of the most critical factors here is biofilm sampling. Sampling in children is even more challenging in particular due to limited space in subgingival areas. We thus focus on the use of paper points for subgingival sampling. This article provides a detailed protocol for oral biofilm sampling of the subgingival sulcus, the mucosa, and saliva in children.
Find related publications in this database (using NLM MeSH Indexing)
Biofilms - growth & development
Child -
Humans -
Microbiota - physiology
Mouth - microbiology

Find related publications in this database (Keywords)
Medicine
Issue 130
Subgingival
mucosal
paper point
sampling
oral biofilm
saliva
microbiota
microbiome
child
orthodontics
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