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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Höfferer, C; Tutkur, D; Fledelius, C; Brand, CL; Alsted, TJ; Damgaard, J; Nishimura, E; Jeppesen, CB; Mautner, SI; Pieber, TR; Sinner, F.
Open flow microperfusion: pharmacokinetics of human insulin and insulin detemir in the interstitial fluid of subcutaneous adipose tissue.
Diabetes Obes Metab. 2015; 17(2):121-127
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Mautner Selma
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Abstract:
To compare the time profile of insulin detemir and human insulin concentrations in the interstitial fluid (ISF) of subcutaneous adipose tissue during constant i.v. infusion and to investigate the relationship between the pharmacokinetics of both insulin molecules in plasma and the ISF of subcutaneous adipose tissue. During a 6-h hyperinsulinaemic-euglycaemic clamp (plasma glucose level 8 mmol/l) human insulin (21 and 42 pmol/min/kg) or insulin detemir (209 and 417 pmol/min/kg) were infused i.v. in eight rats per dose level. Open flow microperfusion (OFM) was used to continuously assess interstitial insulin concentrations in subcutaneous adipose tissue. At the lower infusion rate, insulin detemir appeared significantly later in the ISF than in the plasma (p < 0.05) and also appeared later in the ISF relative to human insulin (p < 0.005). By using OFM we were able to monitor albumin-bound insulin detemir directly in the ISF of subcutaneous tissue and confirm its delayed transendothelial passage to a peripheral site of action. © 2014 John Wiley & Sons Ltd.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Blood Glucose - metabolism
Extracellular Fluid - drug effects
Extracellular Fluid - metabolism
Glucose Clamp Technique -
Hypoglycemic Agents - pharmacokinetics
Hypoglycemic Agents - pharmacology
Insulin Detemir -
Insulin, Long-Acting - pharmacokinetics
Insulin, Long-Acting - pharmacology
Insulin, Regular, Human - pharmacokinetics
Insulin, Regular, Human - pharmacology
Male -
Perfusion - instrumentation
Perfusion - methods
Rats -
Rats, Sprague-Dawley -
Subcutaneous Fat - drug effects
Subcutaneous Fat - pathology
Time Factors -

Find related publications in this database (Keywords)
adipose tissue
animal pharmacology
dose-response relationships
insulin analogues
pharmacokinetics
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