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Eroglu, E; Bischof, H; Charoensin, S; Waldeck-Weiermaier, M; Graier, WF; Malli, R.
Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps.
Methods Mol Biol. 2018; 1747: 23-34.
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Autor/innen der Med Uni Graz:
Bischof Helmut
Eroglu Emrah
Graier Wolfgang
Malli Roland
Waldeck-Weiermair Markus
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Abstract:
Nitric oxide (NO) is a versatile signaling molecule which regulates fundamental cellular processes in all domains of life. However, due to the radical nature of NOit has a very short half-life that makes it challenging to trace its formation, diffusion, and degradation on the level of individual cells. Very recently, we expanded the family of genetically encoded sensors by introducing a novel class of single fluorescent protein-based NOprobes-the geNOps. Once expressed in cells of interest, geNOps selectively respond to NOby fluorescence quench, which enables real-time monitoring of cellular NOsignals. Here, we describe detailed methods suitable for imaging of NOsignals in mammalian cells. This novel approach may facilitate a broad range of studies to (re)investigate the complex NObiochemistry in living cells.

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