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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Eroglu, E; Gottschalk, B; Charoensin, S; Blass, S; Bischof, H; Rost, R; Madreiter-Sokolowski, CT; Pelzmann, B; Bernhart, E; Sattler, W; Hallström, S; Malinski, T; Waldeck-Weiermair, M; Graier, WF; Malli, R.
Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics.
Nat Commun. 2016; 7(3):10623-10623 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bernhart Eva Maria
Bischof Helmut
Blass Sandra
Eroglu Emrah
Gottschalk Benjamin
Graier Wolfgang
Hallström Seth
Madreiter-Sokolowski Corina
Malli Roland
Pelzmann Brigitte
Rost René
Sattler Wolfgang
Waldeck-Weiermair Markus
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Number of Figures: 22
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Abstract:
Nitric oxide () is a free radical with a wide range of biological effects, but practically impossible to visualize in single cells. Here we report the development of novel multicoloured fluorescent quenching-based probes by fusing a bacteria-derived -binding domain close to distinct fluorescent protein variants. These genetically encoded probes, referred to as geNOps, provide a selective, specific and real-time read-out of cellular dynamics and, hence, open a new era of bioimaging. The combination of geNOps with a Ca(2+) sensor allowed us to visualize and Ca(2+) signals simultaneously in single endothelial cells. Moreover, targeting of the probes was used to detect signals within mitochondria. The geNOps are useful new tools to further investigate and understand the complex patterns of signalling on the single (sub)cellular level.
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Animals -
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Endothelial Cells - metabolism
Fluorescent Dyes - metabolism
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Humans -
Luminescent Proteins - metabolism
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