Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Donnerer, J; Liebmann, I.
pERK1/2 immunofluorescence in rat dorsal horn and paraventricular nucleus neurons as a marker for sensitization and inhibition in the pain pathway.
Tissue Cell. 2015; 47(1):55-60
Doi: 10.1016/j.tice.2014.11.003
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Donnerer Josef
- Co-Autor*innen der Med Uni Graz
- Liebmann Ingrid
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- Abstract:
- The aim of the present study was to investigate whether the phosphorylation of ERK1/2 in the rat lumbar dorsal horn and in the parvocellularis part of the paraventricular nucleus can be used to visualize neuronal activity. pERK1/2 fluorescence-immunohistochemistry is specifically suited to mirror neuronal activity in the pain pathway following an acute noxious stimulation. The rat hind paw was either stimulated by noxious heat or by a sequence of mustard oil and noxious heat. Two and 10 min after the thermal stimulation a 3-4-fold increase in cells with pERK1/2 immunoreactivity was observed in lamina I/II of the L3-L5 dorsal horn. The combination of mustard oil with heat led to a 5-6-fold increase in the pERK1/2 signal. The pERK1/2 immunoreactivity in the parvocellularis part of the paraventricular nucleus increased by 2-fold following the heat stimulus, with no further increase following the sequential mustard oil and heat stimulus. A pretreatment with the opioid analgesic morphine or the NMDA antagonist MK-801 markedly attenuated ERK1/2 phosphorylation in both areas of the pain pathway. The present findings support the concept that the pERK1/2 immunofluorescence signal can be used as a quantitative marker for sensitization or inhibition in the pain pathway at spinal and hypothalamic level. Copyright © 2014 Elsevier Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Fluorescent Antibody Technique -
- Hot Temperature -
- Male -
- Mitogen-Activated Protein Kinase 3 - metabolism
- Morphine - administration & dosage
- Mustard Plant - toxicity
- Neurons - enzymology
- Neurons - pathology
- Pain - chemically induced
- Pain - genetics
- Pain - pathology
- Paraventricular Hypothalamic Nucleus - enzymology
- Paraventricular Hypothalamic Nucleus - pathology
- Phosphorylation -
- Plant Oils - toxicity
- Rats -
- Rats, Sprague-Dawley -
- Spinal Cord Dorsal Horn - enzymology
- Spinal Cord Dorsal Horn - pathology
- Find related publications in this database (Keywords)
- ERK1/2 phosphorylation
- Nociception
- Opioid analgesic
- NMDA antagonist
- Spinal cord
- Hypothalamus