Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Siegemund, T; Paulke, BR; Schmiedel, H; Bordag, N; Hoffmann, A; Harkany, T; Tanila, H; Kacza, J; Härtig, W.
Thioflavins released from nanoparticles target fibrillar amyloid beta in the hippocampus of APP/PS1 transgenic mice.
Int J Dev Neurosci. 2006; 24(2-3):195-201
Doi: 10.1016/j.ijdevneu.2005.11.012
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Bordag Natalie
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- Abstract:
- For the delivery of drugs into the brain, the use of nanoparticles as carriers has been described as a promising approach. Here, we prepared nanoparticles as carriers for the model drugs thioflavin T and thioflavin S that bind fibrillar amyloid beta peptides (Abeta). These polymer colloids are composed of a polystyrene core and a degradable PBCA [poly(butyl-2-cyanoacrylate)] shell with a diameter of 90-100nm as shown by dynamic light scattering. Fluorescence spectrophotometric analysis revealed that encapsulated thioflavin T exhibited significantly stronger fluorescence than the free fluorophore. The enzymatic degradation of core-shell nanoparticles, as required in vivo, was shown after their treatment with porcine liver esterase, a non-specific esterase, in vitro. Shells of nanoparticles were dose-dependently degraded while their polystyrene cores remained intact. In the cortices of 7-14 months old APP/PS1 mice with age-dependent beta-amyloidosis, thioflavins selectively targeted fibrillar Abeta after biodegradation-induced release from their nanoparticulate carriers upon intracerebral injection. Collectively, our data suggest that core-shell nanoparticles with controlled degradation in vivo can become versatile tools to trace and clear Abeta in the brain.
- Find related publications in this database (using NLM MeSH Indexing)
- Alzheimer Disease - drug therapy Alzheimer Disease - metabolism Alzheimer Disease - pathology
- Amyloid - metabolism
- Amyloid beta-Protein Precursor - genetics Amyloid beta-Protein Precursor - metabolism
- Animals -
- Disease Models, Animal -
- Dose-Response Relationship, Drug -
- Drug Delivery Systems -
- Hippocampus - drug effects Hippocampus - metabolism
- Mice -
- Mice, Transgenic -
- Nanostructures -
- Neurofibrillary Tangles - drug effects Neurofibrillary Tangles - metabolism
- Protein Binding - drug effects
- Thiazoles - administration & dosage
- Time Factors -
- Find related publications in this database (Keywords)
- Alzheimer's disease
- drug delivery
- drug targeting
- dynamic light scattering
- senile plaque