Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Belic, J; Koch, M; Ulz, P; Auer, M; Gerhalter, T; Mohan, S; Fischereder, K; Petru, E; Bauernhofer, T; Geigl, JB; Speicher, MR; Heitzer, E.
mFast-SeqS as a Monitoring and Pre-screening Tool for Tumor-Specific Aneuploidy in Plasma DNA.
Adv Exp Med Biol. 2016; 924:147-155
Doi: 10.1007/978-3-319-42044-8_28
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Belic Jelena
- Heitzer Ellen
- Co-Autor*innen der Med Uni Graz
- Auer Martina
- Bauernhofer Thomas
- Fischereder Katja
- Geigl Jochen Bernd
- Gerhalter Teresa
- Koch Marina
- Mohan Sumitra
- Petru Edgar
- Speicher Michael
- Ulz Peter
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- Abstract:
- Recent progress in the analysis of cell-free DNA fragments (cell-free circulating tumor DNA, ctDNA) now allows monitoring of tumor genomes by non-invasive means. However, previous studies with plasma DNA from patients with cancer demonstrated highly variable allele frequencies of ctDNA. Comprehensive genome-wide analysis of tumor genomes is greatly facilitated when plasma DNA has increased amounts of ctDNA. In order to develop a fast and cost-effective pre-screening method for the identification of plasma samples suitable for further extensive qualitative analysis, we adapted the recently described FAST-SeqS method. We show that our modified FAST-SeqS method (mFAST-SeqS) can be used as a pre-screening tool for an estimation of the ctDNA percentage. Moreover, since the genome-wide mFAST-SeqS z-scores correlate with the actual tumor content in plasma samples, changes in ctDNA levels associated with response to treatment can be easily monitored without prior knowledge of the genetic composition of tumor samples.
- Find related publications in this database (using NLM MeSH Indexing)
- Aneuploidy -
- Breast Neoplasms - blood
- Breast Neoplasms - diagnosis
- Breast Neoplasms - genetics
- DNA, Neoplasm - blood
- DNA, Neoplasm - genetics
- Female -
- Genome-Wide Association Study -
- HT29 Cells -
- Humans -
- Linear Models -
- MCF-7 Cells -
- Male -
- Mutation -
- Prostatic Neoplasms - blood
- Prostatic Neoplasms - diagnosis
- Prostatic Neoplasms - genetics
- Reproducibility of Results -
- Sensitivity and Specificity -
- Sequence Analysis, DNA - methods
- Find related publications in this database (Keywords)
- Liquid biopsy
- mFAST-SeqS
- Circulating tumor DNA (ctDNA)
- Cancer
- Plasma-Seq
- Copy number alterations (CNA)