Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mayr, C; Wagner, A; Stoecklinger, A; Jakab, M; Illig, R; Berr, F; Pichler, M; Di Fazio, P; Ocker, M; Neureiter, D; Kiesslich, T.
3-Deazaneplanocin A May Directly Target Putative Cancer Stem Cells in Biliary Tract Cancer.
Anticancer Res. 2015; 35(9):4697-4705
Web of Science
PubMed
- Co-Autor*innen der Med Uni Graz
- Pichler Martin
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- Abstract:
- Polycomb repressive complex 2 (PRC2), an epigenetic master regulator, contributes to progression and development of biliary tract cancer (BTC). The present study investigated the effects of the PRC2 inhibitor 3-deazaneplanocin A (DZNep) on BTC cell lines. In vitro effects of DZNep treatment were analyzed for cell viability, gene expression and functional characteristics of cancer stem cell (CSC). DZNep treatment caused a cell line- and dose-dependent decrease in viability. In the EGI-1 cell line, a direct cytotoxic effect was accompanied by mRNA down-regulation of the PRC2 core components, cyclins as well as of CSC-related genes. Furthermore, DZNep affected putative CSCs by reduction of sphere formation and aldehyde dehydrogenase-1-positive cells. The stem cell characteristics of these subpopulations were verified by real-time polymerase chain reaction analysis. Taken together, our results show that DZNep might be a promising pharmacological agent for future therapies regarding BTC. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine - analogs & derivatives
- Adenosine - pharmacology
- Biliary Tract Neoplasms - genetics
- Biliary Tract Neoplasms - pathology
- Biomarkers, Tumor - metabolism
- Cell Death - drug effects
- Cell Line, Tumor -
- Cell Survival - drug effects
- Cyclins - genetics
- Cyclins - metabolism
- Dose-Response Relationship, Drug -
- Gene Expression Regulation, Neoplastic - drug effects
- Humans -
- Neoplastic Stem Cells - drug effects
- Neoplastic Stem Cells - metabolism
- Neoplastic Stem Cells - pathology
- Polycomb Repressive Complex 2 - metabolism
- RNA, Messenger - genetics
- RNA, Messenger - metabolism
- Find related publications in this database (Keywords)
- Biliary tract cancer
- EZH2
- PRC2
- DZNep
- anchorage-independent growth
- cancer stem cells