Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Prüfert, K; Alsheimer, M; Benavente, R; Krohne, G.
The myristoylation site of meiotic lamin C2 promotes local nuclear membrane growth and the formation of intranuclear membranes in somatic cultured cells.
Eur J Cell Biol. 2005; 84(6): 637-646.
Doi: 10.1016/j.ejcb.2004.12.027
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Schild Kristina
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Lamin C2 is a splice product of the mammalian lamin A gene and expressed in primary spermatocytes where it is distributed in the form of discontinuous plaques at the nuclear envelope. We have previously shown that the aminoterminal hexapetide GNAEGR of lamin C2 following the start methionine is essential for its association with the nuclear envelope and that the aminoterminal glycine of the hexapeptide is myristoylated. Here we have analyzed the ultrastructural changes induced in COS-7 and Xenopus A6 cells by overexpressing rat lamin C2 or a human lamin C mutant possessing the lamin C2-specific hexapeptide at its aminoterminus. Both lamins were targeted to the nuclear envelope of mammalian and amphibian cells and induced the formation of intranuclear membranes, whereas wild-type human lamin C and a lamin C2 mutant, that both lack this lipid moiety, did not. Our data indicate that the myristoyl group of lamin C2 has besides its demonstrated role in nuclear envelope association additional functions during spermatogenesis. Our present study complements previously published results where we have shown that the CxxM motif of lamins promotes nuclear membrane growth (Prüfert et al., 2004. J. Cell Sci. 117, 6105-6116).
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- COS Cells -
- Cell Nucleus - metabolism Cell Nucleus - ultrastructure
- Cells, Cultured -
- Cercopithecus aethiops -
- Green Fluorescent Proteins - genetics
- Laminin - chemistry Laminin - genetics Laminin - metabolism
- Meiosis -
- Microscopy, Electron -
- Microscopy, Fluorescence -
- Myristic Acid - chemistry Myristic Acid - metabolism
- Nuclear Envelope - metabolism Nuclear Envelope - ultrastructure
- Recombinant Fusion Proteins - metabolism
- Xenopus laevis -
- Find related publications in this database (Keywords)
- lamin C2
- meiosis
- myristoylation
- nuclear envelope
- membrane growth