Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Prüfert, K; Vogel, A; Krohne, G.
The lamin CxxM motif promotes nuclear membrane growth.
J Cell Sci. 2004; 117(Pt 25): 6105-6116.
Doi: 10.1242/jcs.01532
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- Abstract:
- We analyzed the influence of lamins on nuclear envelope growth in cultured Xenopus A6 cells by the overexpression of human lamin A, Xenopus and zebrafish lamins B2 and Drosophila lamins Dm0 and C as GFP fusion proteins. Lamins containing a CxxM motif in their primary sequence (lamins A, B2, Dm0) induced the formation of lobulated nuclei with multi-membrane-layered, highly folded nuclear membranes and intranuclear membrane assemblies, as observed by electron microscopy. Such morphological alterations were not observed with Drosophila lamin C, a lamin without this motif or with a lamin B2 mutant (B2-SxxM) where the cysteine of the CxxM motif is replaced by a serine. Drosophila lamin C mutants containing a CxxM motif behaved like B-type lamins thus confirming that this tetrapeptide is directly involved in the morphological changes we observed. Nuclear membrane proliferation could also be induced by lamin B2 in COS-7 cells and in zebrafish embryos but not by human lamin A in COS-7 cells. We speculate that the human lamin A is incompletely processed in Xenopus A6 cells and therefore behaves in this cell line like a B-type lamin. Our results indicate that the CxxM motif of B-type lamins has a dual function: it mediates lamin targeting to the inner nuclear membrane thereby promoting nuclear membrane growth.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Motifs -
- Animals -
- COS Cells -
- Cell Membrane - metabolism
- Cell Nucleus - metabolism
- Cell Proliferation -
- Cysteine - chemistry
- Drosophila - metabolism
- Drosophila melanogaster - genetics
- Electrophoresis, Polyacrylamide Gel -
- Green Fluorescent Proteins - metabolism
- Humans -
- Lamin Type A - chemistry Lamin Type A - genetics
- Lamin Type B - chemistry Lamin Type B - genetics
- Microscopy, Electron -
- Microscopy, Fluorescence -
- Mutation -
- Nuclear Envelope - metabolism
- Protein Structure, Tertiary -
- Transcription, Genetic -
- Transfection -
- Xenopus -
- Zebrafish -
- Find related publications in this database (Keywords)
- lamin
- CxxM motif
- nuclear envelope
- intranuclear
- membranes
- nuclear membrane proliferation