Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Meinitzer, A; Kalcher, K; Gartner, G; Halwachs-Baumann, G; Marz, W; Stettin, M.
Drugs and brain death diagnostics: determination of drugs capable of inducing EEG zero line.
Clin Chem Lab Med. 2008; 46(12):1732-1738
Doi: 10.1515/CCLM.2008.335
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Meinitzer Andreas
- Co-Autor*innen der Med Uni Graz
- Baumann Gabriele
- März Winfried
- Stettin Mariana
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- BACKGROUND: Several drugs may affect the diagnosis of brain death by depressing the electroencephalographic signal. Serum levels of these drugs must be below their respective therapeutic ranges. METHODS: A high performance liquid chromatography-based fast and simple method was developed for determination of thiopentone, pentobarbitone, phenobarbitone, methohexital and propofol in serum and validated according to international recommendations. RESULTS: Separation of extracted analytes was performed on a reversed phase column [Agilent Zorbax SB C18, 5 microm, 4.6 x 250 mm; mobile phase 50% 50 mM NaH(2)PO(4) pH 4.6 mixed with 35% (v/v) acetonitrile and 15% (v/v) methanol]. Calibration curves were linear throughout the selected ranges (microg/mL, thiopentone 0.25-50, pentobarbitone 0.25-25, phenobarbitone 2.5-50, methohexital 0.125-2.50, propofol 0.25-5.0). The standard deviations for the regression line, recovery, imprecision and accuracy results were all highly satisfactory. The lower limits of quantification for propofol, thiopentone and pentobarbitone were set at 0.25 microg/mL, for phenobarbitone 2.5 microg/mL, and for methohexital 0.125 microg/mL, which are below the lowest pharmacologically relevant serum concentrations. Intra- and inter-day coefficients of variation were less than 10% throughout as determined with six replicates. CONCLUSIONS: The method presented is suitable for drug monitoring to help enhance the reliability of the diagnosis of brain death.
- Find related publications in this database (using NLM MeSH Indexing)
- Anesthetics, Intravenous -
- Brain Death - blood
- Central Nervous System Depressants - blood
- Chromatography, High Pressure Liquid - methods
- Electroencephalography -
- Humans -
- Hypnotics and Sedatives -
- Methohexital - blood
- Pentobarbital - blood
- Propofol - blood
- Quality Control -
- Thiopental - blood
- Time Factors -
- Find related publications in this database (Keywords)
- brain death diagnosis
- chromatographic method
- drug analysis
- electroencephalography
- validation