Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schraml, E; Fehrer, C; Brunauer, R; Lepperdinger, G; Chesnokova, V; Schauenstein, K.
lin-Sca-1+ cells and age-dependent changes of their proliferation potential are reliant on mesenchymal stromal cells and are leukemia inhibitory factor dependent.
Gerontology. 2008; 54(5):312-323
Doi: 10.1159/000161736
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- Führende Autor*innen der Med Uni Graz
- Schraml Elisabeth
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- Abstract:
- Aging as a process is paralleled by a variety of hematological alterations. Characteristic features are a diminished homeostatic control of blood cell production and a decline in immune functions. It is generally accepted that stromal cells play a basal role in hematopoiesis by providing survival and differentiation signals, by secreting cytokines, or through direct contact with hematopoietic stem cells, thereby supporting the generation and replenishment of hematopoi- etic progenitor cells (HPC). Here we demonstrated that HPC-related colony formation is positively influenced by mesenchymal stromal cells (MSCs) when grown in co-culture, in particular regarding the number of primary granulocyte/macrophage colony-forming units as well as with respect to the average size of the formed colonies. These effects were more pronounced when the MSCs originated from young donors than from old ones. Because leukemia inhibitory factor (LIF) plays an important role during hematopoiesis, properties of lin- Sca-1+ cells and MSCs derived from LIF-deficient mice (LIF-/-) were determined both ex vivo and in vitro. LIF-/- animals contain a significantly reduced number of lin- Sca-1+ cells, nevertheless the replating capacity of LIF-/- HPCs was found to be generally unchanged when compared to those from LIF+/+ animals. However, when cocultured with MSCs, LIF-/- lin- Sca-1+ cells exhibited comparable characteristics to HPCs derived from old wild-type animals.
- Find related publications in this database (using NLM MeSH Indexing)
- Age Factors -
- Analysis of Variance -
- Animals -
- Biopsy -
- Cell Proliferation -
- Coculture Techniques -
- Colony-Forming Units Assay -
- Hematopoiesis - genetics
- Hematopoietic Stem Cells - cytology
- Leukemia Inhibitory Factor - genetics
- Mesoderm - cytology
- Mice -
- Mice, Inbred C57BL -
- Stem Cells - physiology
- Stromal Cells - physiology
- Find related publications in this database (Keywords)
- Hematopoietic stem and progenitor cells
- Mesenchymal stromal cells
- Leukemia inhibitory factor
- Senescence evasion factor