Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bauer, M; Weger, W; Orescovic, I; Hiebaum, EM; Benedicic, C; Lang, U; Pertl, C; Pertl, B.
Fetal microchimerism is not involved in the pathogenesis of lichen sclerosus of the vulva.
PRENATAL DIAG. 2006; 26(2): 175-178.
Doi: 10.1002/pd.1372
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Holzapfel-Bauer Margit
- Pertl Barbara
- Co-Autor*innen der Med Uni Graz
- Lang Uwe
- Magnet Eva Maria Sieglinde
- Weger Wolfgang
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- Abstract:
- OBJECTIVES: The aim of this study was to investigate a possible relationship between fetal cell microchimerism and lichen sclerosus of the vulva. We searched for the presence of male cells and DNA in vulval tissue samples. METHODS: Paraffin-embedded skin biopsy samples from 15 women affected with vulval lichen sclerosus who gave birth to at least one son were analyzed for the presence of microchimeric male cells using fluorescence in situ hybridization (FISH) and fluorescent PCR. We included three lichen sclerosus samples originating from women without male offspring, six vulval specimens without pathological finding originating from autopsies and seven male gingival specimens as controls. RESULTS: Nucleated cells containing Y-chromosome specific sequences were neither detected at any site of the lesions nor in normal vulval specimens by using FISH. These results were confirmed by the use of PCR amplification demonstrating only DNA sequences specific for the X chromosome. No female microchimerism was detected in the male gingival samples. CONCLUSION: Despite the limited number and size of the samples, we conclude that persistent male fetal cells are not involved in the pathogenesis of lichen sclerosus of the vulva, since we consistently could not detect Y-chromosome specific sequences by using two molecular techniques.
- Find related publications in this database (using NLM MeSH Indexing)
- Chimerism -
- Female -
- Fluorescent Dyes - diagnostic use
- Humans - diagnostic use
- In Situ Hybridization, Fluorescence - diagnostic use
- Infant, Newborn - diagnostic use
- Male - diagnostic use
- Polymerase Chain Reaction - diagnostic use
- Pregnancy - diagnostic use
- Skin - chemistry
- Vulva - chemistry
- Vulvar Lichen Sclerosus - diagnosis
- Find related publications in this database (Keywords)
- microchimerism
- lichen sclerosus
- vulva
- FISH
- fluorescent PCR