Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Eberhard, A; Moser, T; Ziegler, L; Vlachos, G; Loibner, M; Bauernhofer, T; Balic, M; Gerger, A; Dandachi, N; Beichler, C; Glawitsch, L; Moser, M; Graf, R; Abuja, PM; Schmitz, M; Krenz, T; Voss, T; Mancarella, D; Heitzer, E.
Evaluation of urinary cfDNA workflows for the molecular of disease
ISCIENCE. 2025; 28(10): 113632
Doi: 10.1016/j.isci.2025.113632
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Eberhard Anna
- Heitzer Ellen
- Co-Autor*innen der Med Uni Graz
- Abuja Peter Michael
- Balic Marija
- Bauernhofer Thomas
- Beichler Christine
- Dandachi Nadia
- Gerger Armin
- Glawitsch Lisa
- Graf Ricarda
- Loibner Martina
- Moser Matthias
- Moser Tina
- Vlachos Georgios
- Ziegler Leandra
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- Abstract:
- Urinary cell-free DNA (ucfDNA) is a promising liquid biopsy analyte obtained by a non-invasive sampling method, particularly valuable in urological cancers due to the proximity of biofluids to the tumor site. However, extremely high fragmentation and low abundance of ucfDNA pose significant analytical challenges. Improper handling and storage can severely affect ucfDNA quality, necessitating optimized pre-analytical workflows for clinical applications. We evaluated multiple pre-analytical workflows, including urine stabilization approaches against unstabilized urine samples. Our results demonstrated that without stabilization, ucfDNA degraded rapidly and minimal quantities remaining after 72 h. In contrast, urine stabilization preserved ucfDNA integrity, enabling successful downstream analyses, including digital PCR and next-generation sequencing, for mutation detection in cancer-related genes and genome wide copy number profiling. These findings underscore the critical role of stabilization for a reliable ucfDNA analysis and highlight its potential in molecular diagnostics as a complementary tool to plasma-based liquid biopsies.