Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schaubeder, JB; Fürk, P; Amering, R; Gsöls, L; Ravn, J; Nypelö, T; Spirk, S.
Deciphering heterogeneous enzymatic surface reactions on xylan using surface plasmon resonance spectroscopy.
Carbohydr Polym. 2024; 337: 122137
Doi: 10.1016/j.carbpol.2024.122137
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Gsöls Lena
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Xylans' unique properties make it attractive for a variety of industries, including paper, food, and biochemical production. While for some applications the preservation of its natural structure is crucial, for others the degradation into monosaccharides is essential. For the complete breakdown, the use of several enzymes is required, due to its structural complexity. In fact, the specificity of enzymatically-catalyzed reactions is guided by the surface, limiting or regulating accessibility and serving structurally encoded input guiding the actions of the enzymes. Here, we investigate enzymes at surfaces rich in xylan using surface plasmon resonance spectroscopy. The influence of diffusion and changes in substrate morphology is studied via enzyme surface kinetics simulations, yielding reaction rates and constants. We propose kinetic models, which can be applied to the degradation of multilayer biopolymer films. The most advanced model was verified by its successful application to the degradation of a thin film of polyhydroxybutyrate treated with a polyhydroxybutyrate-depolymerase. The herein derived models can be employed to quantify the degradation kinetics of various enzymes on biopolymers in heterogeneous environments, often prevalent in industrial processes. The identification of key factors influencing reaction rates such as inhibition will contribute to the quantification of intricate dynamics in complex systems.
- Find related publications in this database (using NLM MeSH Indexing)
- Xylans - chemistry, metabolism
- Surface Plasmon Resonance - methods
- Kinetics - administration & dosage
- Surface Properties - administration & dosage
- Find related publications in this database (Keywords)
- Enzymatic degradation
- Xylanases
- Reaction kinetics
- Multilayer thin films
- Polyhydroxybutyrate
- Biopolymers