Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Duller, S; Vrbancic, S; Szydłowski, Ł; Mahnert, A; Blohs, M; Predl, M; Kumpitsch, C; Zrim, V; Högenauer, C; Kosciolek, T; Schmitz, RA; Eberhard, A; Dragovan, M; Schmidberger, L; Zurabischvili, T; Weinberger, V; Moser, AM; Kolb, D; Pernitsch, D; Mohammadzadeh, R; Kühnast, T; Rattei, T; Moissl-Eichinger, C.
Targeted isolation of Methanobrevibacter strains from fecal samples expands the cultivated human archaeome.
Nat Commun. 2024; 15(1): 7593
Doi: 10.1038/s41467-024-52037-7
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- Führende Autor*innen der Med Uni Graz
- Duller Stefanie
- Moissl-Eichinger Christine
- Co-Autor*innen der Med Uni Graz
- Blohs Marcus
- Dragovan Melanie
- Eberhard Anna
- Hoegenauer Christoph
- Kolb Dagmar
- Kühnast Torben Wilhelm
- Kumpitsch Christina Sarah
- Mahnert Alexander
- Mohammadzadeh Rokhsareh
- Pernitsch Dominique
- Weinberger Viktoria
- Zrim Verena
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- Abstract:
- Archaea are vital components of the human microbiome, yet their study within the gastrointestinal tract (GIT) is limited by the scarcity of cultured representatives. Our study presents a method for the targeted enrichment and isolation of methanogenic archaea from human fecal samples. The procedure combines methane breath testing, in silico metabolic modeling, media optimization, FACS, dilution series, and genomic sequencing through Nanopore technology. Additional analyzes include the co-cultured bacteriome, comparative genomics of archaeal genomes, functional comparisons, and structure-based protein function prediction of unknown differential traits. Successful establishment of stable archaeal cultures from 14 out of 16 fecal samples yielded nine previously uncultivated strains, eight of which are absent from a recent archaeome genome catalog. Comparative genomic and functional assessments of Methanobrevibacter smithii and Candidatus Methanobrevibacter intestini strains from individual donors revealed features potentially associated with gastrointestinal diseases. Our work broadens available archaeal representatives for GIT studies, and offers insights into Candidatus Methanobrevibacter intestini genomes' adaptability in critical microbiome contexts.
- Find related publications in this database (using NLM MeSH Indexing)
- Methanobrevibacter - genetics, isolation & purification, metabolism
- Humans - administration & dosage
- Feces - microbiology
- Gastrointestinal Microbiome - genetics
- Genome, Archaeal - administration & dosage
- Methane - metabolism
- Phylogeny - administration & dosage
- Adult - administration & dosage
- Male - administration & dosage
- Female - administration & dosage
- Gastrointestinal Tract - microbiology