Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zacharias, M; Konjic, S; Kratochwill, N; Absenger, G; Terbuch, A; Jost, PJ; Wurm, R; Lindenmann, J; Kashofer, K; Gollowitsch, F; Gorkiewicz, G; Brcic, L.
Expanding Broad Molecular Reflex Testing in Non-Small Cell Lung Cancer to Squamous Histology.
Cancers (Basel). 2024; 16(5): 903
Doi: 10.3390/cancers16050903
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- Führende Autor*innen der Med Uni Graz
- Brcic Luka
- Zacharias Martin
- Co-Autor*innen der Med Uni Graz
- Absenger Gudrun
- Gollowitsch Franz
- Gorkiewicz Gregor
- Jost Philipp
- Kashofer Karl
- Konjic Selma
- Kratochwill Nikolaus
- Lindenmann Jörg
- Terbuch Angelika
- Wurm Robert
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- Abstract:
- Due to the success story of biomarker-driven targeted therapy, most NSCLC guidelines agree that molecular reflex testing should be performed in all cases with non-squamous cell carcinoma (non-SCC). In contrast, testing recommendations for squamous cell carcinoma (SCC) vary considerably, specifically concerning the exclusion of patients of certain age or smoking status from molecular testing strategies. We performed a retrospective single-center study examining the value of molecular reflex testing in an unselected cohort of 316 consecutive lung SCC cases, tested by DNA- and RNA-based next-generation sequencing (NGS) at our academic institution between 2019 and 2023. Clinicopathological data from these cases were obtained from electronic medical records and correlated with sequencing results. In 21/316 (6.6%) cases, we detected an already established molecular target for an approved drug. Among these were seven cases with an EGFR mutation, seven with a KRAS G12C mutation, four with an ALK fusion, two with an EGFR fusion and one with a METex14 skipping event. All patients harboring a targetable alteration were >50 years of age and most of them had >15 pack-years, questioning restrictive molecular testing strategies. Based on our real-world data, we propose a reflex testing workflow using DNA- and RNA-based NGS that includes all newly diagnosed NSCLC cases, irrespective of histology, but also irrespective of age or smoking status.
- Find related publications in this database (Keywords)
- lung cancer
- squamous cell carcinoma
- molecular testing
- next-generation sequencing