Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Monedeiro, F; Milanowski, M; Ratiu, IA; Zmysłowski, H; Ligor, T; Buszewski, B.
VOC Profiles of Saliva in Assessment of Halitosis and Submandibular Abscesses Using HS-SPME-GC/MS Technique.
Molecules. 2019; 24(16):
Doi: 10.3390/molecules24162977
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- Co-Autor*innen der Med Uni Graz
- Monedeiro-Milanowski Maciej
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- Abstract:
- Halitosis and submandibular abscesses are examples of mouth-related diseases with the possible bacterial origin. Salivary volatile organic compounds (VOCs) are potential biomarkers of them, once they can be addressed as metabolites of bacterial activity. Healthy patients (n = 15), subjects with submandibular abscesses located in fascial deep space (n = 10), and subjects with halitosis (n = 5) were enrolled in the study. Saliva samples were subjected to headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometry (GC/MS) analysis. A total number of 164 VOCs was detected by the developed methodology, 23 specific for halitosis and 41 for abscess. Halitosis' profiles were characterized by a larger number of sulfur compounds, while for abscess they had a higher variety of alcohols, aldehydes, and hydrocarbons-biomarkers of inflammatory processes. Principal components analysis allowed visualization of clusters formed according to the evaluated conditions. Kruskal-Wallis test indicated that 39 VOCs presented differentiated responses between the studied groups, with statistical relevance (p < 0.05). Random forest was applied, and a prediction model based on eight VOCs (2-butanone, methyl thioacetate, 2-methylbutanoic acid, S-methyl pentanethioate, dimethyl tetrasulfide, indolizine, pentadecane, and octadecanal) provided 100% of sensitivity, 82% of specificity, and 91% of balanced accuracy, indicating the specific presence of submandibular abscess.
- Find related publications in this database (using NLM MeSH Indexing)
- Abscess - diagnosis, metabolism, pathology
- Adult - administration & dosage
- Aged - administration & dosage
- Alcohols - classification, isolation & purification
- Aldehydes - classification, isolation & purification
- Biomarkers - analysis
- Case-Control Studies - administration & dosage
- Dentate Gyrus - metabolism, pathology
- Diagnosis, Differential - administration & dosage
- Female - administration & dosage
- Gas Chromatography-Mass Spectrometry - administration & dosage
- Halitosis - diagnosis, metabolism, pathology
- Humans - administration & dosage
- Hydrocarbons - classification, isolation & purification
- Male - administration & dosage
- Mandible - metabolism, pathology
- Middle Aged - administration & dosage
- Principal Component Analysis - administration & dosage
- Saliva - chemistry
- Sensitivity and Specificity - administration & dosage
- Solid Phase Microextraction - methods
- Sulfur Compounds - classification, isolation & purification
- Volatile Organic Compounds - administration & dosage
- Find related publications in this database (Keywords)
- saliva
- halitosis
- submandibular abscess
- VOCs
- GC
- MS
- diagnosis