Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Dichtl, K; Klugherz, I; Greimel, H; Luxner, J; Köberl, J; Friedl, S; Steinmetz, I; Leitner, E.
A head-to-head comparison of three MALDI-TOF mass spectrometry systems with 16S rRNA gene sequencing.
J Clin Microbiol. 2023; e0191322
Doi: 10.1128/jcm.01913-22
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Dichtl Karl
- Co-Autor*innen der Med Uni Graz
- Friedl Simone
- Greimel Hanna
- Klugherz Isabel
- Köberl Julian Allan
- Leitner-Meyer Eva
- Luxner Josefa
- Steinmetz Ivo
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized diagnostics in culture-based microbiology. Commonly used MALDI-TOF MS systems in clinical microbiology laboratories are MALDI Biotyper (Bruker Daltonics) and Vitek MS (bioMérieux), but recently the new EXS2600 (Zybio) has been launched. This study aimed to evaluate the performance of the three devices by comparing the results to 16S rRNA gene sequencing. A set of 356 previously collected difficult-to-identify bacteria was tested in parallel with the three systems. Only the direct smear method and simple formic acid extraction were applied. Valid results were achieved for 98.6%, 94.4%, and 93.3% of all isolates by MALDI Biotyper, EXS2600, and Vitek MS, respectively. Of all valid results, agreement with sequencing data was achieved in 98.9%, 98.5%, and 99.7% by MALDI Biotyper, EXS2600, and Vitek MS, respectively. Considering only the isolates with valid measurements at the single-species level, misidentification rates were 0%, 2.6%, and 1.1% for MALDI Biotyper, EXS2600, and Vitek MS, respectively. Apart from minor performance differences, our data demonstrate that the three systems provide comparable results and are suitable for use in medical diagnostic laboratories.
- Find related publications in this database (Keywords)
- MALDI-TOF
- mass spectrometry
- identification
- Biotyper
- Vitek MS
- EXS2600