Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Skok, K; Gradišnik, L; Čelešnik, H; Milojević, M; Potočnik, U; Jezernik, G; Gorenjak, M; Sobočan, M; Takač, I; Kavalar, R; Maver, U.
MFUM-BrTNBC-1, a Newly Established Patient-Derived Triple-Negative Breast Cancer Cell Line: Molecular Characterisation, Genetic Stability, and Comprehensive Comparison with Commercial Breast Cancer Cell Lines.
Cells. 2021; 11(1):
Doi: 10.3390/cells11010117
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Skok Kristijan
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- Abstract:
- Triple-negative breast cancer (TNBC) is a breast cancer (BC) subtype that accounts for approximately 15-20% of all BC cases. Cancer cell lines (CLs) provide an efficient way to model the disease. We have recently isolated a patient-derived triple-negative BC CL MFUM-BrTNBC-1 and performed a detailed morphological and molecular characterisation and a comprehensive comparison with three commercial BC CLs (MCF-7, MDA-MB-231, MDA-MB-453). Light and fluorescence microscopy were used for morphological studies; immunocytochemical staining for hormone receptor, p53 and Ki67 status; RNA sequencing, qRT-PCR and STR analysis for molecular characterisation; and biomedical image analysis for comparative phenotypical analysis. The patient tissue-derived MFUM-BrTNBC-1 maintained the primary triple-negative receptor status. STR analysis showed a stable and unique STR profile up to the 6th passage. MFUM-BrTNBC-1 expressed EMT transition markers and displayed changes in several cancer-related pathways (MAPK, Wnt and PI3K signalling; nucleotide excision repair; and SWI/SNF chromatin remodelling). Morphologically, MFUM-BrTNBC-1 differed from the commercial TNBC CL MDA-MB-231. The advantages of MFUM-BrTNBC-1 are its isolation from a primary tumour, rather than a metastatic site; good growth characteristics; phenotype identical to primary tissue; complete records of origin; a unique identifier; complete, unique STR profile; quantifiable morphological properties; and genetic stability up to (at least) the 6th passage.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line, Tumor - administration & dosage
- Cell Proliferation - administration & dosage
- Female - administration & dosage
- Humans - administration & dosage
- Signal Transduction - administration & dosage
- Triple Negative Breast Neoplasms - genetics
- Find related publications in this database (Keywords)
- breast cancer cell lines
- MFUM-BrTNBC-1
- MCF-7
- MDA-MB-231
- MDA-MB-453
- hormonal receptors
- triple-negative breast cancer