Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zelzer, S; Meinitzer, A; Enko, D; Herrmann, M; Goessler, W.
Development of a two-dimensional liquid chromatography-tandem mass-spectrometry method for the determination of vitamin D2 in mushrooms.
Anal Bioanal Chem. 2022; 414(26): 7565-7572.
Doi: 10.1007/s00216-022-04281-3
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- Führende Autor*innen der Med Uni Graz
- Zelzer Sieglinde
- Co-Autor*innen der Med Uni Graz
- Enko Dietmar
- Herrmann Markus
- Meinitzer Andreas
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- Abstract:
- Different foods, especially mushrooms, are a valuable source of vitamin D2. However, published concentrations in mushrooms show large variabilities. One reason for this is certainly the high biological variability caused by growth conditions, and another could also be found in the analytical methodology. Therefore, this study aimed to develop a sensitive and highly selective two-dimensional liquid chromatography mass spectrometry (LC-MS/MS) method for vitamin D2 analysis in mushrooms. After validation, the method was applied to four different mushroom species. The developed method with a one-step extraction procedure showed a limit of detection of 0.01 µg vitamin D2/g dry mass (DM), a limit of quantification of 0.05 µg vitamin D2/g DM, and recovery rates between 87.6 and 94.8%. The total run time including the re-equilibration of the columns for the next injection was 7.5 min. After adding increased concentrations of pure substance to Pleurotus ostreatus, Lentinula edodes, and brown and white button mushrooms the standard addition plot showed excellent correlation coefficients (R2) of > 0.9994. Mean vitamin D2 concentrations were observed at 0.122 ± 0.007, 0.074 ± 0.005, 0.099 ± 0.007, and 0.073 ± 0.005 µg/g DM. The coefficient of variation (CV) was between 5.1 and 7.6%. This well-optimized, sensitive LC-MS/MS method, with a fast and simple sample preparation and a short run time, can be applied to future studies especially in different mushroom species with variable growing conditions. This will improve our knowledge about the vitamin D2 content in mushrooms.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromatography, Liquid - administration & dosage
- Ergocalciferols - analysis
- Tandem Mass Spectrometry - methods
- Food - administration & dosage
- Find related publications in this database (Keywords)
- Vitamin D2
- LC-MS
- MS
- Mushrooms
- Standard addition
- Irradiation