Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kessler, HH; Pruller, F; Hardt, M; Stelzl, E; Foderl-Hobenreich, E; Pailer, S; Lueger, A; Kreuzer, P; Zatloukal, K; Herrmann, M.
Identification of contagious SARS-CoV-2 infected individuals by Roche's Rapid Antigen Test
CLIN CHEM LAB MED. 2022;
Doi: 10.1515/cclm-2021-1276
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Herrmann Markus
- Kessler Harald
- Co-Autor*innen der Med Uni Graz
- Föderl-Höbenreich Esther
- Hardt Melina
- Lueger Andreas
- Pailer Sabine
- Prüller Florian
- Stelzl Evelyn
- Zatloukal Kurt
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- Abstract:
- Objectives Rapid antigen tests (RAT) can provide valuable information on the presence or absence SARS-CoV-2 within 15 min without the need of a laboratory. The analytical and diagnostic characteristics of available RATs has led to the question whether they can safely distinguish between infectious and non-infectious patients in an acute care setting. Methods Three nasopharyngeal swabs for the analysis by RAT, reverse transcriptase real time polymerase chain reaction (RT-qPCR), and a cell culture based infection assay were collected from 67 patients that presented to the emergency department of the University Hospital of Graz (Austria). The first swab was used for on-site RAT testing in the emergency department using the Roche SARS-CoV-2 RAT. The second swab was sent to the central laboratory of the hospital for RT-qPCR with two independent methods (Cepheid Xpert(R) Xpress SARS-CoV-2 assay and Roche Cobas SARS-CoV-2 Test) and repeat RAT testing using the same commercial test. With the third swab a cell culture-based infection assay was performed. Results The RATs performed from independent samples showed substantial agreement (Cohen's-kappa: 0.73, p<0.001). All patients with a positive RAT had positive RT-qPCR with cycle threshold (ct) values 25 did not induce cytopathic effects that were related to SARS-CoV-2. The infection assays from four RAT-negative patients showed cytopathic effects that were induced by other pathogens. Conclusions The SARS-CoV-2 RAT from Roche Diagnostics is a valuable tool for managing symptomatic patients. RAT-negative patients may be regarded as non-contagious.
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