Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kaufmann, L; Syedbasha, M; Vogt, D; Hollenstein, Y; Hartmann, J; Linnik, JE; Egli, A.
An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers.
J Vis Exp. 2017; (130):
Doi: 10.3791/55833
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- Führende Autor*innen der Med Uni Graz
- Kaufmann Lukas
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- Abstract:
- Antibody titers are commonly used as surrogate markers for serological protection against influenza and other pathogens. Detailed knowledge of antibody production pre- and post-vaccination is required to understand vaccine-induced immunity. This article describes a reliable point-by-point protocol to determine influenza-specific antibody titers. The first protocol describes a method to specify the antigen amounts required for hemagglutination, which standardizes the concentrations for subsequent usage in the second protocol (hemagglutination assay, HA assay). The second protocol describes the quantification of influenza-specific antibody titers against different viral strains by using a serial dilution of human serum or cell culture supernatants (hemagglutination inhibition assay, HI assay). As an applied example, we show the antibody response of a healthy cohort, which received a trivalent inactivated influenza vaccine. Additionally, the cross-reactivity between the different influenza viruses is shown and methods to minimize cross-reactivity by using different types of animal red blood cells (RBCs) are explained. The discussion highlights advantages and disadvantages of the presented assays and how the determination of influenza-specific antibody titers can improve the understanding of vaccine-related immunity.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Viral - blood
- Antibody Specificity -
- Cross Reactions - immunology
- Hemagglutination Inhibition Tests - methods
- Humans -
- Influenza Vaccines - immunology
- Influenza, Human - blood
- Influenza, Human - immunology
- Influenza, Human - prevention & control
- Influenza, Human - virology
- Find related publications in this database (Keywords)
- Medicine
- Issue 130
- Hemagglutination inhibition assay
- screening
- titer
- antibody
- influenza A
- influenza B
- H1N1
- H3N2
- cross-reactivity
- titration
- antigen