Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Leopold, C; Duta-Mare, M; Sachdev, V; Goeritzer, M; Maresch, LK; Kolb, D; Reicher, H; Wagner, B; Stojakovic, T; Ruelicke, T; Haemmerle, G; Hoefler, G; Sattler, W; Kratky, D.
Hepatocyte-specific lysosomal acid lipase deficiency protects mice from diet-induced obesity but promotes hepatic inflammation.
Biochim Biophys Acta Mol Cell Biol Lipids. 2019; 1864(4):500-511
Doi: 10.1016/j.bbalip.2019.01.007
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Kratky Dagmar
- Leopold Christina
- Co-Autor*innen der Med Uni Graz
- Duta-Mare Madalina-Cristina
- Göritzer Madeleine
- Hinteregger Helga
- Höfler Gerald
- Kolb Dagmar
- Sachdev Vinay
- Sattler Wolfgang
- Stojakovic Tatjana
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Lysosomal acid lipase (LAL) hydrolyzes cholesteryl esters (CE) and triglycerides (TG) to generate fatty acids (FA) and cholesterol. LAL deficiency (LAL-D) in both humans and mice leads to hepatomegaly, hypercholesterolemia, and shortened life span. Despite its essential role in lysosomal neutral lipid catabolism, the cell type-specific contribution of LAL to disease progression is still elusive. To investigate the role of LAL in the liver in more detail and to exclude the contribution of LAL in macrophages, we generated hepatocyte-specific LAL-deficient mice (Liv-Lipa-/-) and fed them either chow or high fat/high cholesterol diets (HF/HCD). Comparable to systemic LAL-D, Liv-Lipa-/- mice were resistant to diet-induced obesity independent of food intake, movement, and energy expenditure. Reduced body weight gain was mainly due to reduced white adipose tissue depots. Furthermore, Liv-Lipa-/- mice exhibited improved glucose clearance during glucose and insulin tolerance tests compared to control mice. Analysis of hepatic lipid content revealed a massive reduction of TG, whereas CE concentrations were markedly increased, leading to CE crystal formation in the livers of Liv-Lipa-/- mice. Elevated plasma transaminase activities, increased pro-inflammatory cytokines and chemokines as well as hepatic macrophage infiltration indicated liver inflammation. Our data provide evidence that hepatocyte-specific LAL deficiency is sufficient to alter whole-body lipid and energy homeostasis in mice. We conclude that hepatic LAL plays a pivotal role by preventing liver damage and maintaining lipid and energy homeostasis, especially during high lipid availability. Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.
- Find related publications in this database (Keywords)
- LAL-D
- Cholesteryl ester storage disease
- Wolman disease
- Fibrosis
- Liver damage
- Lipid