Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Obasi, A; Nwachukwu, S; Ugoji, E; Kohler, C; Göhler, A; Balau, V; Pfeifer, Y; Steinmetz, I.
Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae from Pharmaceutical Wastewaters in South-Western Nigeria.
Microb Drug Resist. 2017; 23(8): 1013-1018.
Doi: 10.1089/mdr.2016.0269
Web of Science
PubMed
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- Steinmetz Ivo
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- Abstract:
- Emergence and spread of Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases (ESBLs) present a major threat to public health. In this study, we characterized β-lactam-resistant K. pneumoniae isolates from six wastewater samples obtained from two pharmaceutical industries located in Lagos and Ogun States, Nigeria. Bacteria were isolated by using MacConkey agar; species identification and antibacterial susceptibility testing were performed by Vitek 2. Etest was used for ESBL phenotype confirmation. The presence of β-lactamase genes was investigated by PCR and sequencing. Bacterial strain typing was done by XbaI-macrorestriction and subsequent pulsed-field gel electrophoresis (PFGE) as well as multilocus sequence typing (MLST). Thirty-five bacterial species were isolated from the six samples; among them, we identified seven K. pneumoniae isolates with resistance to β-lactams and co-resistance to fluoroquinolones, aminoglycosides, and folate pathway inhibitors. The ESBL phenotype was confirmed in six K. pneumoniae isolates that harbored ESBL genes blaCTX-M-15 (n = 5), blaSHV-2 (n = 1), and blaSHV-12 (n = 1). PFGE and MLST analysis revealed five clones belonging to four sequence types (ST11, ST15, ST37, ST101), and clone K. pneumoniae-ST101 was present in the wastewater samples from two different pharmaceutical industries. Additionally performed conjugation assays confirmed the location of β-lactamase genes on conjugative plasmids. This is the first confirmation of K. pneumoniae isolates producing CTX-M-15-ESBL from pharmaceutical wastewaters in Nigeria. The co-resistance observed might be a reflection of the different drugs produced by these industries. Continuous surveillance of the environmental reservoirs of multidrug-resistant bacteria is necessary to prevent their further spread.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Bacterial Agents - pharmacology
- Bacterial Proteins - metabolism
- Bacterial Typing Techniques - methods
- Drug Industry - methods
- Drug Resistance, Multiple, Bacterial - drug effects
- Klebsiella pneumoniae - drug effects
- Klebsiella pneumoniae - metabolism
- Molecular Epidemiology - methods
- Nigeria -
- Waste Water - microbiology
- beta-Lactamases - metabolism
- beta-Lactams - pharmacology
- Find related publications in this database (Keywords)
- CTX-M-15
- SHV-12
- SHV-2
- multidrug resistance
- conjugative plasmids