Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bast, A; Kubis, H; Holtfreter, B; Ribback, S; Martin, H; Schreiner, HC; Dominik, MJ; Breitbach, K; Dombrowski, F; Kocher, T; Steinmetz, I.
NADPH Oxidase Contributes to Resistance against Aggregatibacter actinomycetemcomitans-Induced Periodontitis in Mice.
Infect Immun. 2017; 85(2):
Doi: 10.1128/IAI.00849-16
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Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- Aggregatibacter actinomycetemcomitans is a Gram-negative commensal bacterium of the oral cavity which has been associated with the pathogenesis of periodontitis with severe alveolar bone destruction. The role of host factors such as reactive oxygen and nitrogen intermediates in periodontal A. actinomycetemcomitans infection and progression to periodontitis is still ill-defined. Therefore, this study aimed to analyze the role of NADPH oxidase and inducible nitric oxide synthase (iNOS) in a murine model of A. actinomycetemcomitans-induced periodontitis. NADPH oxidase-deficient (gp91phoxknockout [KO]), iNOS-deficient (iNOS KO), and C57BL/6 wild-type mice were orally infected with A. actinomycetemcomitans and analyzed for bacterial colonization at various time points. Alveolar bone mineral density and alveolar bone volume were quantified by three-dimensional micro-computed tomography, and the degree of tissue inflammation was calculated by histological analyses. At 5 weeks after infection, A. actinomycetemcomitans persisted at significantly higher levels in the murine oral cavities of infected gp91phoxKO mice than in those of iNOS KO and C57BL/6 mice. Concomitantly, alveolar bone mineral density was significantly lower in all three infected groups than in uninfected controls, but with the highest loss of bone density in infected gp91phoxKO mice. Only infected gp91phoxKO mice revealed significant loss of alveolar bone volume and enhanced inflammatory cell infiltration, as well as an increased number of osteoclasts. Our results indicate that NADPH oxidase is important to control A. actinomycetemcomitans infection in the murine oral cavity and to prevent subsequent alveolar bone destruction and osteoclastogenesis. Copyright © 2017 American Society for Microbiology.
- Find related publications in this database (using NLM MeSH Indexing)
- Aggregatibacter actinomycetemcomitans -
- Alveolar Bone Loss - diagnostic imaging
- Alveolar Bone Loss - pathology
- Animals -
- Bacterial Load -
- Bone Density -
- Disease Models, Animal -
- Disease Resistance -
- Female -
- Host-Pathogen Interactions -
- Membrane Glycoproteins - deficiency
- Membrane Glycoproteins - genetics
- Mice -
- Mice, Knockout -
- NADPH Oxidase 2 -
- NADPH Oxidases - deficiency
- NADPH Oxidases - genetics
- NADPH Oxidases - metabolism
- Nitric Oxide Synthase Type II - genetics
- Nitric Oxide Synthase Type II - metabolism
- Osteoclasts - metabolism
- Periodontitis - diagnosis
- Periodontitis - genetics
- Periodontitis - metabolism
- Periodontitis - microbiology
- Find related publications in this database (Keywords)
- Aggregatibacter actinomycetemcomitans
- NADPH oxidase
- alveolar bone loss
- bone mineral density
- micro-computed tomography
- periodontal disease