Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Assmann, G; Werner, C; Herrmann, M; Momber, D; Krenn, T; Lothschütz, D; Fischer, L; Hess, S; Ketter, R; Feiden, W; Jung, S; Herrmann, W; Schubert, J; Pfreundschuh, M; Widmann, T.
Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity.
Clin Chem Lab Med. 2009; 47(10):1271-1276
Doi: 10.1515/CCLM.2009.258
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Herrmann Markus
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Analysis of cerebrospinal fluid (CSF) to discriminate between benign and malignant conditions is of fundamental importance for the physician and the patient because of the differential therapeutic options and resulting morbidity and mortality. Most human tumours demonstrate increased telomerase activity (TA). Recent technical advances in the detection of TA allow for sensitive and specific detection within 4 h. Thus, the detection of TA is suitable for routine clinical testing. This study examines TA in cellular proteins in CSF from 111 patients compared to cytomorphological and laboratory examination. A positive result for TA in cellular proteins of CSF was correlated significantly with Meningeosis neoplastica, but not with non-malignant conditions. Telomerase was not detected in CSF supernatant, despite positive results in cellular proteins from identical patients. Furthermore, a 48-h time delay during the pre-analytic processing is not critical for detection of TA detection in native CSF when stored at room temperature. We conclude that TA is a promising marker for the detection of Meningeosis neoplastica and warrants further study.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Line -
- Child -
- Feasibility Studies -
- Female -
- Humans -
- Male -
- Meningitis - cerebrospinal fluid
- Meningitis - diagnosis
- Meningitis - enzymology
- Middle Aged -
- Polymerase Chain Reaction -
- Reproducibility of Results -
- Sensitivity and Specificity -
- Telomerase - genetics
- Telomerase - metabolism
- Time Factors -
- Find related publications in this database (Keywords)
- cerebrospinal fluid
- Meningeosis neoplastica
- real-time quantitative telomere repeat amplification protocol
- telomerase activity