Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Cekauskas, A; Bruns, H; Manikas, M; Herr, I; Gross, ML; Zorn, M; Jankevicius, F; Strupas, K; Schemmer, P.
Sulforaphane decreases kidney injury after transplantation in rats: role of mitochondrial damage.
Ann Transplant. 2013; 18(3-4):488-496
Doi: 10.12659/AOT.884013
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- Führende Autor*innen der Med Uni Graz
- Schemmer Peter
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- Abstract:
- Sulforaphane is a naturally occuring antioxidative and anti-inflammatory isothiocyanat. In this study, its impact on experimental kidney transplantation was evaluated. Male Brown Norway rats (n=112) were used as experimental animals. Donor kidneys were harvested and stored for 12 hours in HTK-solution at 4°C. D,L-Sulforaphane (4.4 mg/kg BW; 0.2ml) or normal saline (0.2 ml) was given i.v. to the recipients 24 and 1 hour before, and 6 hours after transplantation. Recipients were nephrectomized bilaterally and subsequently transplantation was performed. After 6 and 48 hours, biopsies were taken and processed for light and electron microscopy. Graft function was monitored using serum values of creatinine and BUN after 6 and 24 hours. Quantitative real-time PCR was used to detect differences in SOD2-gene expression after 6 hours and apoptotic activity was detected after 6 hours using propidium iodide flow cytometry. Recipient preconditioning improved reperfusion damage index from 12.8±1.6 in controls to 8.8±1.8 (p<0.001). Serum levels of creatinine and BUN decreased from 4.29±0.25 mg/dl and 119±23 mg/dl in controls to 3.65±0.7 mg/dl and 81±19 mg/dl (p<0.05). The number of severely injured tubules decreased (p<0.05). Apoptotic activity was increased in SFN-treated rats. Mitochondrial microstructure was better preserved after SFN, while SOD 2 gene expression increased (p<0.05). SFN ameliorates ischemia/reperfusion injury after KTx, most likely through anti-oxidative effects.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antioxidants - pharmacology
- Antioxidants - therapeutic use
- Apoptosis - drug effects
- Isothiocyanates - pharmacology
- Isothiocyanates - therapeutic use
- Kidney - drug effects
- Kidney - metabolism
- Kidney - pathology
- Kidney Transplantation - methods
- Male -
- Mitochondria - drug effects
- Mitochondria - metabolism
- Mitochondria - pathology
- Rats -
- Reperfusion Injury - drug therapy
- Reperfusion Injury - metabolism
- Reperfusion Injury - pathology
- Superoxide Dismutase - genetics
- Superoxide Dismutase - metabolism
- Find related publications in this database (Keywords)
- kidney transplantation
- ischemia/reperfusion
- sulforaphane