Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sun, X; Göhler, A; Heller, KJ; Neve, H.
The ltp gene of temperate Streptococcus thermophilus phage TP-J34 confers superinfection exclusion to Streptococcus thermophilus and Lactococcus lactis.
Virology. 2006; 350(1):146-157
Doi: 10.1016/j.virol.2006.03.001
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Göhler Andre
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The ltp gene, located within the lysogeny module of temperate Streptococcus thermophilus phage TP-J34, has been shown to be expressed in lysogenic strain S. thermophilus J34. It codes for a lipoprotein, as demonstrated by inhibition of cleavage of the signal sequence by globomycin. Exposure of Ltp on the surface of Lactococcus lactis protoplasts bearing a plasmid-encoded copy of ltp has been demonstrated by immunogold labeling and electron microscopy. Expression of ltp in prophage- and plasmid-cured S. thermophilus J34-6f interfered with TP-J34 infection. While plating efficiency was reduced by a factor of about 40 and lysis of strain J34-6f in liquid medium was delayed considerably, phage adsorption was not affected at all. Intracellular accumulation of phage DNA was shown to be inhibited by Ltp. This indicates interference of Ltp with infection at the stage of triggering DNA release and injection into the cell, indicating a role of Ltp in superinfection exclusion. Expression of ltp in L. lactis Bu2-60 showed that the same superinfection exclusion mechanism was strongly effective against phage P008, a member of the lactococcal 936 phage species: no plaque-formation was detectable with even 10(9) phage per ml applied, and lysis in liquid medium did not occur. In Lactococcus also, Ltp apparently inhibited phage DNA release and/or injection. Ltp appears to be a member of a family of small, secreted proteins with a 42 amino acids repeat structure encoded by genes of Gram-positive bacteria. Some of these homologous genes are part of the genomes of prophages.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- DNA, Viral -
- Escherichia coli -
- Gene Expression Regulation, Viral -
- Genes, Viral - genetics
- Lactococcus lactis - ultrastructure Lactococcus lactis - virology
- Lipoproteins - genetics Lipoproteins - metabolism
- Lysogeny - physiology
- Molecular Sequence Data -
- Streptococcus Phages - genetics Streptococcus Phages - physiology
- Streptococcus thermophilus - virology
- Superinfection - virology
- Viral Proteins - genetics Viral Proteins - metabolism
- Find related publications in this database (Keywords)
- temperate S. thermophilus phage TP-J34
- ltp gene
- lipoprotein
- superinfection exclusion
- Lactococcus lactis phage p008