Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Renner, W; Jahrbacher, R; Marx-Neuhold, E; Tischler, S; Zulus, B.
A novel exonuclease (TaqMan) assay for rapid haptoglobin genotyping.
Clin Chem Lab Med. 2016; 54(5):781-783
Doi: 10.1515/cclm-2015-0586
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Renner Wilfried
- Co-Autor*innen der Med Uni Graz
- Sternat Renate
- Tischler Simone
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- Abstract:
- Haptoglobin is an acute-phase binding protein that scavenges free hemoglobin. The human haptoglobin gene (HP) is polymorphic with two main alleles, haptoglobin allele 1 (Hp1) and haptoglobin allele 2 (Hp2). The smaller Hp1 allele features no duplication and consists of four exons, whereas the larger Hp2 allele, containing a 1.7 kb duplication, consists of six exons, with the fifth and sixth being highly homologous to exons 3 and 4 of Hp1. We designed an exonuclease (TaqMan) assay targeting single nucleotide differences between the homologous regions of Hp1 and Hp2. The assay contained one probe specifically binding to a site in intron 4 of Hp2, and another probe binding equally to intron 4 of Hp1 and intron 6 of Hp2. Measurement of post-PCR fluorescence allowed unambiguous discrimination of HP genotypes. Comparison with genotypes obtained by a method based upon allele-specific primers yielded fully corresponding results. The new HP genotyping method is fast, reliable, does not require real-time instruments and may be especially useful for high-throughput genotyping.
- Find related publications in this database (using NLM MeSH Indexing)
- Alleles -
- Exonucleases - metabolism
- Fluorescence -
- Genotype -
- Haptoglobins - genetics
- Humans -
- Polymerase Chain Reaction -
- Find related publications in this database (Keywords)
- epidemiology
- genotyping
- haptoglobin