Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Boehnke, K; Iversen, PW; Schumacher, D; Lallena, MJ; Haro, R; Amat, J; Haybaeck, J; Liebs, S; Lange, M; Schäfer, R; Regenbrecht, CR; Reinhard, C; Velasco, JA.
Assay Establishment and Validation of a High-Throughput Screening Platform for Three-Dimensional Patient-Derived Colon Cancer Organoid Cultures.
J Biomol Screen. 2016; 21(9):931-941
Doi: 10.1177/1087057116650965
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- Co-Autor*innen der Med Uni Graz
- Haybäck Johannes
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- Abstract:
- The application of patient-derived three-dimensional culture systems as disease-specific drug sensitivity models has enormous potential to connect compound screening and clinical trials. However, the implementation of complex cell-based assay systems in drug discovery requires reliable and robust screening platforms. Here we describe the establishment of an automated platform in 384-well format for three-dimensional organoid cultures derived from colon cancer patients. Single cells were embedded in an extracellular matrix by an automated workflow and subsequently self-organized into organoid structures within 4 days of culture before being exposed to compound treatment. We performed validation of assay robustness and reproducibility via plate uniformity and replicate-experiment studies. After assay optimization, the patient-derived organoid platform passed all relevant validation criteria. In addition, we introduced a streamlined plate uniformity study to evaluate patient-derived colon cancer samples from different donors. Our results demonstrate the feasibility of using patient-derived tumor samples for high-throughput assays and their integration as disease-specific models in drug discovery. © 2016 Society for Laboratory Automation and Screening.
- Find related publications in this database (using NLM MeSH Indexing)
- Antineoplastic Agents - isolation & purification
- Antineoplastic Agents - therapeutic use
- Cell Culture Techniques - methods
- Cell Line, Tumor -
- Colonic Neoplasms - drug therapy
- Drug Discovery - methods
- Drug Screening Assays, Antitumor - methods
- High-Throughput Screening Assays - methods
- Humans -
- Organoids - growth & development
- Organoids - pathology
- Spheroids, Cellular - drug effects
- Find related publications in this database (Keywords)
- cell-based assays
- oncology
- assay validation
- patient-derived organoid culture