Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bedenić, B; Sardelić, S; Luxner, J; Bošnjak, Z; Varda-Brkić, D; Lukić-Grlić, A; Mareković, I; Frančula-Zaninović, S; Krilanović, M; Šijak, D; Grisold, A; Zarfel, G.
Molecular characterization of class b carbapenemases in advanced stage of dissemination and emergence of class d carbapenemases in Enterobacteriaceae from Croatia.
Infect Genet Evol. 2016; 43(3):74-82
Doi: 10.1016/j.meegid.2016.05.011
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- Co-Autor*innen der Med Uni Graz
- Grisold Andrea
- Luxner Josefa
- Zarfel Gernot
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- Abstract:
- Carbapenemases involved in acquired carbapenem resistance in Enterobacteriaceae belong to Ambler class A serin β-lactamases, class B metallo-β-lactamases (MBL) or class D OXA-48-like β-lactamases. The aim of the present study was to analyse the molecular epidemiology and the mechanisms and routes of spread of class B and class D carbapenemases in Croatia. In total 68 isolates were analyzed. Antibiotic susceptibility was determined by broth microdilution method. PCR was used to detect antibiotic-resistance genes. Genotyping was performed by rep-PCR and MLST. Sixty-five isolates were found to harbour VIM-1 carbapenemase, seven of which were positive also for NDM-1, while two strains harboured only NDM-1. OXA-48 was detected in three isolates, two of which coproduced VIM-1. Thirty-six strains possessed additional CTX-M-15 β-lactamase whereas 64 were positive for TEM-1. CMY was found in 18 Citrobacter freundii isolates and DHA-1 in one Enterobacter cloacae isolate. Four different plasmid-incompatibility groups were found: A/C, L/M, N and FIIAs. Unlike C. freundii and E. cloacae, Klebsiella pneumoniae showed high diversity of rep-PCR patterns. E. cloacae and C. freundii predominantly belonged to one large clone which was allocated to ST105 and ST24, respectively. Three different types of carbapenemases were identified showing the complexity of CRE in Croatia. Copyright © 2016 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Carbapenems - pharmacology
- Croatia -
- Drug Resistance, Bacterial -
- Enterobacteriaceae - classification
- Enterobacteriaceae - drug effects
- Enterobacteriaceae - genetics
- Enterobacteriaceae - isolation & purification
- Enterobacteriaceae Infections - epidemiology
- Enterobacteriaceae Infections - microbiology
- Genotyping Techniques -
- Humans -
- Microbial Sensitivity Tests -
- Multilocus Sequence Typing -
- Phylogeny -
- beta-Lactamases - classification
- beta-Lactamases - genetics
- Find related publications in this database (Keywords)
- Enterobacteriaceae
- VIM-1
- NDM-1
- OXA-48
- Carbapenems