Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fengler, VH; Macheiner, T; Kessler, SM; Czepukojc, B; Gemperlein, K; Müller, R; Kiemer, AK; Magnes, C; Haybaeck, J; Lackner, C; Sargsyan, K.
Susceptibility of Different Mouse Wild Type Strains to Develop Diet-Induced NAFLD/AFLD-Associated Liver Disease.
PLoS One. 2016; 11(5):e0155163-e0155163
Doi: 10.1371/journal.pone.0155163
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- Führende Autor*innen der Med Uni Graz
- Fengler Vera
- Sargsyan Karine
- Co-Autor*innen der Med Uni Graz
- Haybäck Johannes
- Kessler Sonja
- Lackner Karoline
- Macheiner Tanja
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- Abstract:
- Although non-alcoholic and alcoholic fatty liver disease have been intensively studied, concerning pathophysiological mechanisms are still incompletely understood. This may be due to the use of different animal models and resulting model-associated variation. Therefore, this study aimed to compare three frequently used wild type mouse strains in their susceptibility to develop diet-induced features of non-alcoholic/alcoholic fatty liver disease. Fatty liver disease associated clinical, biochemical, and histological features in C57BL/6, CD-1, and 129Sv WT mice were induced by (i) high-fat diet feeding, (ii) ethanol feeding only, and (iii) the combination of high-fat diet and ethanol feeding. Hepatic and subcutaneous adipose lipid profiles were compared in CD-1 and 129Sv mice. Additionally hepatic fatty acid composition was determined in 129Sv mice. In C57BL/6 mice dietary regimens resulted in heterogeneous hepatic responses, ranging from pronounced steatosis and inflammation to a lack of any features of fatty liver disease. Liver-related serum biochemistry showed high deviations within the regimen groups. CD-1 mice did not exhibit significant changes in metabolic and liver markers and developed no significant steatosis or inflammation as a response to dietary regimens. Although 129Sv mice showed no weight gain, this strain achieved most consistent features of fatty liver disease, apparent from concentration alterations of liver-related serum biochemistry as well as moderate steatosis and inflammation as a result of all dietary regimens. Furthermore, the hepatic lipid profile as well as the fatty acid composition of 129Sv mice were considerably altered, upon feeding the different dietary regimens. Accordingly, diet-induced non-alcoholic/alcoholic fatty liver disease is most consistently promoted in 129Sv mice compared to C57BL/6 and CD-1 mice. As a conclusion, this study demonstrates the importance of genetic background of used mouse strains for modeling diet-induced non-alcoholic/alcoholic fatty liver disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Alanine Transaminase - metabolism
- Animals -
- Aspartate Aminotransferases - metabolism
- Biomarkers - metabolism
- Cholesterol - metabolism
- Diet, High-Fat - adverse effects
- Dietary Fats - administration & dosage
- Disease Models, Animal -
- Disease Susceptibility -
- Ethanol - administration & dosage
- Fatty Acids, Nonesterified - metabolism
- Fatty Liver, Alcoholic - etiology
- Fatty Liver, Alcoholic - genetics
- Fatty Liver, Alcoholic - metabolism
- Fatty Liver, Alcoholic - pathology
- Liver - metabolism
- Liver - pathology
- Liver Function Tests -
- Male -
- Mice -
- Mice, 129 Strain -
- Mice, Inbred C57BL -
- Mice, Inbred Strains -
- Non-alcoholic Fatty Liver Disease - etiology
- Non-alcoholic Fatty Liver Disease - genetics
- Non-alcoholic Fatty Liver Disease - metabolism
- Non-alcoholic Fatty Liver Disease - pathology
- Species Specificity -
- Subcutaneous Fat - metabolism
- Subcutaneous Fat - pathology
- Triglycerides - metabolism
- Weight Gain -