Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Radović, B; Vujić, N; Leopold, C; Schlager, S; Goeritzer, M; Patankar, JV; Korbelius, M; Kolb, D; Reindl, J; Wegscheider, M; Tomin, T; Birner-Gruenberger, R; Schittmayer, M; Groschner, L; Magnes, C; Diwoky, C; Frank, S; Steyrer, E; Du, H; Graier, WF; Madl, T; Kratky, D.
Lysosomal acid lipase regulates VLDL synthesis and insulin sensitivity in mice.
Diabetologia. 2016; 59(8):1743-1752
Doi: 10.1007/s00125-016-3968-6
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Kratky Dagmar
- Radovic Branislav
- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Frank Sasa
- Göritzer Madeleine
- Graier Wolfgang
- Groschner Lukas
- Kolb Dagmar
- Korbelius Melanie
- Leopold Christina
- Madl Tobias
- Schittmayer-Schantl Matthias
- Schlager Stefanie
- Steyrer Ernst
- Tomin Tamara
- Vujic Nemanja
- Wegscheider Martin
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Lysosomal acid lipase (LAL) hydrolyses cholesteryl esters and triacylglycerols (TG) within lysosomes to mobilise NEFA and cholesterol. Since LAL-deficient (Lal (-/-) ) mice suffer from progressive loss of adipose tissue and severe accumulation of lipids in hepatic lysosomes, we hypothesised that LAL deficiency triggers alternative energy pathway(s). We studied metabolic adaptations in Lal (-/-) mice. Despite loss of adipose tissue, Lal (-/-) mice show enhanced glucose clearance during insulin and glucose tolerance tests and have increased uptake of [(3)H]2-deoxy-D-glucose into skeletal muscle compared with wild-type mice. In agreement, fasted Lal (-/-) mice exhibit reduced glucose and glycogen levels in skeletal muscle. We observed 84% decreased plasma leptin levels and significantly reduced hepatic ATP, glucose, glycogen and glutamine concentrations in fed Lal (-/-) mice. Markedly reduced hepatic acyl-CoA concentrations decrease the expression of peroxisome proliferator-activated receptor α (PPARα) target genes. However, treatment of Lal (-/-) mice with the PPARα agonist fenofibrate further decreased plasma TG (and hepatic glucose and glycogen) concentrations in Lal (-/-) mice. Depletion of hepatic nuclear factor 4α and forkhead box protein a2 in fasted Lal (-/-) mice might be responsible for reduced expression of microsomal TG transfer protein, defective VLDL synthesis and drastically reduced plasma TG levels. Our findings indicate that neither activation nor inactivation of PPARα per se but rather the availability of hepatic acyl-CoA concentrations regulates VLDL synthesis and subsequent metabolic adaptations in Lal (-/-) mice. We conclude that decreased plasma VLDL production enhances glucose uptake into skeletal muscle to compensate for the lack of energy supply.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cholesterol, VLDL - genetics
- Cholesterol, VLDL - metabolism
- Female -
- Glucose - metabolism
- Insulin Resistance - genetics
- Insulin Resistance - physiology
- Lipolysis - genetics
- Lipolysis - physiology
- Liver - metabolism
- Lysosomes - metabolism
- Male -
- Mice -
- Sterol Esterase - deficiency
- Sterol Esterase - genetics
- Sterol Esterase - metabolism
- Triglycerides - metabolism
- Find related publications in this database (Keywords)
- Glucose tolerance
- Lipolysis
- Lysosomes
- VLDL