Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Trung, TT; Hetzer, A; Topfstedt, E; Göhler, A; Limmathurotsakul, D; Wuthiekanun, V; Peacock, SJ; Steinmetz, I.
Improved culture-based detection and quantification of Burkholderia pseudomallei from soil.
Trans R Soc Trop Med Hyg. 2011; 105(6):346-351
Doi: 10.1016/j.trstmh.2011.03.004
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Steinmetz Ivo
- Co-Autor*innen der Med Uni Graz
- Göhler Andre
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- Abstract:
- Environmental surveillance of the Gram-negative soil bacterium Burkholderia pseudomallei, the aetiological agent of melioidosis, is important in order to define human populations and livestock at risk of acquiring the infection. This study aimed to develop a more sensitive method for the detection of B. pseudomallei from soil samples in endemic areas compared with the currently used culture method based on soil dispersion in water. We report the development of a new protocol that involves soil dispersion in a polyethylene glycol (PEG) and sodium deoxycholate (DOC) solution to increase the yield of viable B. pseudomallei from soil samples. Comparative testing of soil samples from Northeast Thailand covering a wide range of B. pseudomallei concentrations demonstrated a significantly higher recovery (P<0.0001) of B. pseudomallei colony-forming units by the new method compared with the conventional method. The data indicate that using the detergents PEG and DOC not only results in a higher recovery of viable B. pseudomallei but also results in a shift in the bacterial species recovered from soil samples. Future studies on the geographical distribution and population structure of B. pseudomallei in soil are likely to benefit from the new protocol described here. Copyright © 2011 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Burkholderia pseudomallei - genetics
- Burkholderia pseudomallei - isolation & purification
- Colony Count, Microbial -
- Female -
- Humans -
- Male -
- Melioidosis - epidemiology
- Melioidosis - microbiology
- Soil Microbiology -
- Soil Microbiology - epidemiology
- Find related publications in this database (Keywords)
- Burkholderia pseudomallei
- Melioidosis
- Soil
- Culturing
- Polyethylene glycol
- Sodium deoxycholate