Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bast, A; Erttmann, SF; Walther, R; Steinmetz, I.
Influence of iNOS and COX on peroxiredoxin gene expression in primary macrophages.
Free Radic Biol Med. 2010; 49(12):1881-1891
Doi: 10.1016/j.freeradbiomed.2010.09.015
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- Peroxiredoxins (Prxs) are a family of multifunctional antioxidant thiol-dependent peroxidases. This study aimed to examine the regulatory mechanisms of Prx gene expression in murine bone marrow-derived macrophages (BMMs) using standardized serum-free conditions. Stimulation with LPS and IFNγ increased mRNA levels of Prx 1, 2, 4, 5, and 6 in BMMs of both C57BL/6 and BALB/c mice, with Prx 1, 2, 4, and 6 more strongly induced in C57BL/6 BMMs. Further investigations on signaling pathways in C57BL/6 BMMs demonstrated that up-regulation of Prx 5 and 6 by LPS and IFNγ was associated with the activation of multiple protein kinases, most notably JAK2, PI3K, and p38 MAPK. Our experiments also revealed a contribution of inducible NO synthase-derived nitric oxide to the increase in Prx 1, 2, 4, and 6 mRNA expression, whereas NADPH oxidase-derived superoxide was not involved. Furthermore, we could show that LPS- and IFNγ-induced gene expression of Prx 6 was also regulated in an NO-independent manner by cyclooxygenases and prostaglandin E(2). Taken together our results indicate a possible role for Prxs in defense mechanisms of activated macrophages against oxidative stress during inflammation or infection. Copyright © 2010 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cyclooxygenase 1 - metabolism
- Cyclooxygenase 2 - metabolism
- Dinoprostone - secretion
- Enzyme Activation -
- Interferon-gamma - pharmacology
- Intracellular Signaling Peptides and Proteins - metabolism
- Lipopolysaccharides - pharmacology
- Macrophages - metabolism
- Mice -
- Mice, Inbred BALB C -
- Mice, Inbred C57BL -
- NADPH Oxidases - metabolism
- Nitric Oxide Synthase Type II - metabolism
- Oxidative Stress -
- Peroxiredoxin VI - genetics
- Peroxiredoxin VI - metabolism
- Peroxiredoxins - genetics
- Peroxiredoxins - metabolism
- Reactive Oxygen Species - metabolism
- Up-Regulation -
- Find related publications in this database (Keywords)
- Cyclooxygenase
- iNOS
- JAK2
- Macrophage
- MAPK
- NADPH oxidase
- Peroxiredoxin
- PI3K
- Prostaglandin
- Free radicals