Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zaloga, J; Pöttler, M; Leitinger, G; Friedrich, RP; Almer, G; Lyer, S; Baum, E; Tietze, R; Heimke-Brinck, R; Mangge, H; Dörje, F; Lee, G; Alexiou, C.
Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting.
Eur J Pharm Biopharm. 2016; 101(3):152-162
Doi: 10.1016/j.ejpb.2016.01.017
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Almer Gunter
- Leitinger Gerd
- Mangge Harald
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- Abstract:
- In this work we present a new formulation of superparamagnetic iron oxide nanoparticles (SPIONs) for magnetic drug targeting. The particles were reproducibly synthesized from current good manufacturing practice (cGMP) - grade substances. They were surface coated using fatty acids as anchoring molecules for human serum albumin. We comprehensively characterized the physicochemical core-shell structure of the particles using sophisticated methods. We investigated biocompatibility and cellular uptake of the particles using an established flow cytometric method in combination with microwave-plasma assisted atomic emission spectroscopy (MP-AES). The cytotoxic drug mitoxantrone was adsorbed on the protein shell and we showed that even in complex media it is slowly released with a close to zero order kinetics. We also describe an in vitro proof-of-concept assay in which we clearly showed that local enrichment of this SPION-drug conjugate with a magnet allows site-specific therapeutic effects. Copyright © 2016. Published by Elsevier B.V.
- Find related publications in this database (using NLM MeSH Indexing)
- Biocompatible Materials - chemistry
- Cell Line, Tumor -
- Chemistry, Pharmaceutical - methods
- Drug Delivery Systems -
- Ferric Compounds - chemistry
- Humans -
- Jurkat Cells -
- Magnetics - methods
- Microscopy, Atomic Force - methods
- Mitoxantrone - chemistry
- Nanoparticles - chemistry
- Serum Albumin - chemistry
- Find related publications in this database (Keywords)
- Superparamagnetic iron oxide nanoparticles
- Good manufacturing practice
- Magnetic drug targeting
- Human serum albumin