Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zelzer, S; Mangge, H; Oberreither, R; Bernecker, C; Gruber, HJ; Prüller, F; Fauler, G.
Oxidative stress: Determination of 4-hydroxy-2-nonenal by gas chromatography/mass spectrometry in human and rat plasma.
Free Radic Res. 2015; 49(10):1233-1238
Doi: 10.3109/10715762.2015.1059936
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Mangge Harald
- Zelzer Sieglinde
- Co-Autor*innen der Med Uni Graz
- Bernecker Claudia
- Fauler Günter
- Gruber Hans-Jürgen
- Prüller Florian
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- Abstract:
- The lipid peroxidation product 4-hydroxynonenal (HNE) is a biomarker of oxidative stress which is essentially involved in the pathophysiology of many diseases. The analysis of HNE is challenging because this aldehyde is extremely reactive and thus unstable. Hence, we adopted a gas chromatography-mass spectrometry (GC-MS) method based on derivatization of HNE with pentafluorobenzyl-hydroxylamine-HCl followed by trimethylsilylation to trimethylsilyl ethers. Ions representative for a negative ion chemical ionization mode were recorded at m/z = 152 for HNE and at m/z = 162 for the deuterated analogon (HNE-d11) as internal standard. This excellent stable and precise GC-MS method was carefully validated for HNE, and showed good linearity (r(2) = 0.998), and high specificity and sensitivity. Within-day precisions were 4.4-6.1% and between-day precisions were 5.2-10.2%. Accuracies were between 99% and 104% for the whole calibration range (2.5-250 nmol/L) of HNE. To examine the versatility of this modified GC-MS method, we analyzed HNE in ethylenediaminetetraacetic acid (EDTA) plasma in a well-defined collective of migraine patients; recently published. The results underline our former observations that women with migraine are afflicted with increased levels of HNE. Patients with thyroidal dysfunction showed no significant HNE alterations. This was confirmed by normal HNE EDTA plasma levels in hyper- und hypothyroid Sprague-Dawley rats. Taken together, the GC-MS method presented herein is of excellent quality to record oxidative stress-related bioactive HNE levels. This is important for a reorientation of oxidative stress analytics in other human diseases first of atherosclerosis and cancer.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aldehydes - blood
- Aldehydes - chemistry
- Animals -
- Biomarkers -
- Case-Control Studies -
- Female -
- Gas Chromatography-Mass Spectrometry - methods
- Humans -
- Hydroxylamines - chemistry
- Hyperthyroidism - blood
- Hypothyroidism - blood
- Lipid Peroxidation -
- Migraine Disorders - blood
- Rats -
- Rats, Sprague-Dawley -
- Sensitivity and Specificity -
- Trimethylsilyl Compounds - analysis
- Find related publications in this database (Keywords)
- 4-hydroxy-2-nonenal
- GC-MS
- oxidative stress
- human plasma
- Sprague-Dawley rats