Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Szabo, S; Wögenstein, KL; Österreicher, CH; Guldiken, N; Chen, Y; Doler, C; Wiche, G; Boor, P; Haybaeck, J; Strnad, P; Fuchs, P.
Epiplakin attenuates experimental mouse liver injury by chaperoning keratin reorganization.
J Hepatol. 2015; 62(6):1357-1366
Doi: 10.1016/j.jhep.2015.01.007
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- Co-Autor*innen der Med Uni Graz
- Haybäck Johannes
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- Abstract:
- Epiplakin is a member of the plakin protein family and exclusively expressed in epithelial tissues where it binds to keratins. Epiplakin-deficient (Eppk1(-/-)) mice displayed no obvious spontaneous phenotype, but their keratinocytes showed a faster keratin network breakdown in response to stress. The role of epiplakin in the stressed liver remained to be elucidated. Wild-type (WT) and Eppk1(-/-) mice were subjected to common bile duct ligation (CBDL) or fed with a 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-containing diet. The importance of epiplakin during keratin reorganization was assessed in primary hepatocytes. Our experiments revealed that epiplakin is expressed in hepatocytes and cholangiocytes, and binds to keratin 8 (K8) and K18 via multiple domains. In several liver stress models epiplakin and K8 genes displayed identical expression patterns and transgenic K8 overexpression resulted in elevated hepatic epiplakin levels. After CBDL and DDC treatment, Eppk1(-/-) mice developed a more pronounced liver injury and their livers contained larger amounts of hepatocellular keratin granules, indicating impaired disease-induced keratin network reorganization. In line with these findings, primary Eppk1(-/-) hepatocytes showed increased formation of keratin aggregates after treatment with the phosphatase inhibitor okadaic acid, a phenotype which was rescued by the chemical chaperone trimethylamine N-oxide (TMAO). Finally, transfection experiments revealed that Eppk1(-/-) primary hepatocytes were less able to tolerate forced K8 overexpression and that TMAO treatment rescued this phenotype. Our data indicate that epiplakin plays a protective role during experimental liver injuries by chaperoning disease-induced keratin reorganization. Copyright © 2015 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Autoantigens - genetics
- Autoantigens - metabolism
- Chemical and Drug Induced Liver Injury - metabolism
- Chemical and Drug Induced Liver Injury - pathology
- Chemical and Drug Induced Liver Injury - prevention & control
- Female -
- Hepatocytes - drug effects
- Hepatocytes - metabolism
- Hepatocytes - pathology
- Humans -
- Keratin-18 - metabolism
- Keratin-8 - genetics
- Keratin-8 - metabolism
- Liver - injuries
- Liver - metabolism
- Liver - pathology
- Male -
- Methylamines - pharmacology
- Mice -
- Mice, Inbred C57BL -
- Mice, Knockout -
- Mice, Transgenic -
- Molecular Chaperones - genetics
- Molecular Chaperones - metabolism
- Protein Aggregates -
- Proteolysis -
- Stress, Physiological -
- Up-Regulation -
- Find related publications in this database (Keywords)
- Chaperone
- Common bile duct ligation
- DDC
- Keratin aggregates
- Plakins