Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Siwetz, M; Sundl, M; Kolb, D; Hiden, U; Herse, F; Huppertz, B; Gauster, M.
Placental fractalkine mediates adhesion of THP-1 monocytes to villous trophoblast.
Histochem Cell Biol. 2015; 143(6):565-574
Doi: 10.1007/s00418-014-1304-0
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- Führende Autor*innen der Med Uni Graz
- Gauster Martin
- Siwetz Monika
- Co-Autor*innen der Med Uni Graz
- Hiden Ursula
- Huppertz Berthold
- Kolb Dagmar
- Sundl Monika
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- Abstract:
- The chemokine fractalkine (CX3CL1) recently attracted increasing attention in the field of placenta research due to its dual nature, acting both as membrane-bound and soluble forms. While the membrane-bound form mediates flow-resistant adhesion of leukocytes to endothelial and epithelial cells via its corresponding receptor CX3CR1, the soluble form arises from metalloprotease-dependent shedding and bears chemoattractive activity for monocytes, natural killer cells and T cells. In human placenta, fractalkine is expressed at the apical microvillous plasma membrane of the syncytiotrophoblast, which may enable close physical contact with circulating maternal leukocytes. Based on these observations, we tested the hypothesis that fractalkine mediates adhesion of monocytes to the villous trophoblast. Forskolin-induced differentiation and syncytialization of the trophoblast cell line BeWo was accompanied with a substantial upregulation in fractalkine expression and led to increased adhesion of the monocyte cell line THP-1, which preferentially bound to syncytia. Blocking as well as silencing of the fractalkine receptor CX3CR1 proved involvement of the fractalkine/CX3CR1 system in adherence of THP-1 monocytes to villous trophoblast. Pre-incubation of THP-1 monocytes with human recombinant fractalkine as well as silencing of CX3CR1 expression in THP-1 monocytes significantly impaired their adherence to BeWo cells and primary term trophoblasts. The present study suggests fractalkine as another candidate among the panel of adhesion molecules enabling stable interaction between leukocytes and the syncytiotrophoblast.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Adhesion -
- Cells, Cultured -
- Chemokine CX3CL1 - genetics
- Chemokine CX3CL1 - metabolism
- Female -
- Humans -
- Monocytes - cytology
- Monocytes - metabolism
- Placenta - chemistry
- Placenta - cytology
- Pregnancy -
- Trophoblasts - cytology
- Trophoblasts - metabolism
- Find related publications in this database (Keywords)
- Placental fractalkine
- Chemokine
- Trophoblast
- Monocyte
- Adhesion