Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Strenger, V; Caselli, E; Lautenschlager, I; Schwinger, W; Aberle, SW; Loginov, R; Gentili, V; Nacheva, E; DiLuca, D; Urban, C.
Detection of HHV-6-specific mRNA and antigens in PBMCs of individuals with chromosomally integrated HHV-6 (ciHHV-6).
Clin Microbiol Infect. 2014; 20(10):1027-1032
Doi: 10.1111/1469-0691.12639
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- Führende Autor*innen der Med Uni Graz
- Strenger Volker
- Co-Autor*innen der Med Uni Graz
- Schwinger Wolfgang
- Urban Ernst-Christian
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- Abstract:
- After inheritance of chromosomally integrated HHV-6 (ciHHV-6), viral DNA is found in every nucleated cell. The prevalence of ciHHV-6 is estimated to be 0.2-5% of humans. There are conflicting data on the potential for replication, possibly leading to clinical implications. We analysed peripheral blood mononuclear cells (PBMCs) from individuals with ciHHV-6 proven by fluorescence in situ hybridization (FISH) for HHV-6-specific mRNA (U94, U42, U22) and antigens by means of reverse transcription PCR and an indirect immunoperoxidase staining. U94 transcripts indicative of latent infection were detected in six (54.5%) out of 11 individuals at least once. Transcripts indicative of lytic infection (i.e. U42 and U22) were detected in four (36.4%) out of 11 individuals at least once. HHV-6 antigen was detected in seven (70%) out of 10 individuals at least once. The presence of viral mRNA and proteins supports virus gene expression from ciHHV-6, which may lead to virus replication. Considering the properties of active HHV-6 infection together with obvious replicative activity in individuals with ciHHV-6, pathophysiological effects leading to clinical consequences of chromosomally integrated viral DNA might be considered. © 2014 The Authors Clinical Microbiology and Infection © 2014 European Society of Clinical Microbiology and Infectious Diseases.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Antigens, Viral - genetics
- Antigens, Viral - metabolism
- Child -
- Chromosomes, Human - virology
- Female -
- Herpesvirus 6, Human - genetics
- Herpesvirus 6, Human - immunology
- Humans -
- Leukocytes, Mononuclear - virology
- Male -
- Middle Aged -
- Molecular Diagnostic Techniques - methods
- RNA, Messenger - genetics
- RNA, Viral - genetics
- Reverse Transcriptase Polymerase Chain Reaction -
- Roseolovirus Infections - blood
- Roseolovirus Infections - diagnosis
- Roseolovirus Infections - virology
- Sensitivity and Specificity -
- Virus Integration -
- Young Adult -
- Find related publications in this database (Keywords)
- Chromosomal integration
- herpesvirus
- HHV-6
- lytic infection
- mRNA
- viral antigen
- viral replication