Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Waltl-Patankar, S; Patankar, J; Fauler, G; Nusshold, C; Ullen, A; Eibinger, G; Wintersperger, A; Kratky, D; Malle, E; Sattler, W.
25-Hydroxycholesterol regulates cholesterol homeostasis in the murine CATH.a neuronal cell line.
Neurosci Lett. 2013; 539(4):16-21
Doi: 10.1016/j.neulet.2013.01.014
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- Führende Autor*innen der Med Uni Graz
- Sattler Wolfgang
- Waltl Sabine Evelyn
- Co-Autor*innen der Med Uni Graz
- Eibinger Gerald
- Fauler Günter
- Kratky Dagmar
- Malle Ernst
- Nusshold Christoph
- Patankar Jay Vasant
- Üllen Andreas
- Wintersperger Andrea
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- Abstract:
- Aberrant oxysterol biosynthesis is implicated in the pathogenesis of neurodegenerative diseases. During the present study we have investigated the effects of exogenously added 25-hydroxycholesterol (25-HC) on transcription of cholesterol biosynthetic genes, sterol-regulatory element binding protein (SREBP) processing and cholesterol biosynthesis in the murine CATH.a neuronal cell line. A single i.p. injection of lipopolysaccharide resulted in robust induction of cholesterol 25-hydroxylase mRNA and protein levels in brains of treated mice. In vitro, 25-HC upregulated the transcription of ATP-binding cassette transporter A1 (ABCA1) and (to a lesser extent) apolipoprotein E (apoE) in CATH.a neurons. Cholesterol biosynthetic gene expression (squalene synthase, HMG-CoA synthase, HMG-CoA reductase, and SREBP2) was downregulated by 25-HC. 25-HC also significantly attenuated proteolytic processing of SREBP2. Finally, 25-HC downregulated cholesterol biosynthesis in CATH.a neurons. Our results demonstrate that 25-HC is a potent effector oxysterol of neuronal cholesterol homeostasis.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Line -
- Cholesterol - biosynthesis Cholesterol - genetics
- Homeostasis -
- Hydroxycholesterols - metabolism Hydroxycholesterols - pharmacology
- Lipopolysaccharides - pharmacology
- Mice -
- Neurons - drug effects Neurons - metabolism
- RNA, Messenger - metabolism
- Steroid Hydroxylases - genetics Steroid Hydroxylases - metabolism
- Sterol Regulatory Element Binding Proteins - genetics Sterol Regulatory Element Binding Proteins - metabolism
- Transcription, Genetic -
- Find related publications in this database (Keywords)
- 25-Hydroxycholesterol
- Cholesterol 25-hydroxylase
- Oxysterol
- Endotoxin
- Neuroinflammation
- Neurons