Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sreckovic, I; Birner-Gruenberger, R; Obrist, B; Stojakovic, T; Scharnagl, H; Holzer, M; Scholler, M; Philipose, S; Marsche, G; Lang, U; Desoye, G; Wadsack, C.
Distinct composition of human fetal HDL attenuates its anti-oxidative capacity.
Biochim Biophys Acta. 2013; 1831(4):737-746
Doi: 10.1016/j.bbalip.2012.12.015
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Sreckovic Ivana
- Wadsack Christian
- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Desoye Gernot
- Holzer Michael
- Lang Uwe
- Marsche Gunther
- Obrist Britta
- Scharnagl Hubert
- Scholler Monika
- Sonia Philipose Sonia Philipose
- Stojakovic Tatjana
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- In human high-density lipoprotein (HDL) represents the major cholesterol carrying lipoprotein class in cord blood, while cholesterol is mainly carried by low-density lipoprotein in maternal serum. Additionally, to carrying cholesterol, HDL also associates with a range of proteins as cargo. We tested the hypothesis that fetal HDL carries proteins qualitatively and quantitatively different from maternal HDL. These differences then contribute to distinct HDL functionality in both circulations. Shotgun proteomics and biochemical analyses were used to assess composition/function of fetal and maternal HDL isolated from uncomplicated human pregnancies at term of gestation. The pattern of analyzed proteins that were statistically elevated in fetal HDL (apoE, proteins involved in coagulation, transport processes) suggests a particle characteristic for the light HDL2 sub-fraction. In contrast, proteins that were enriched in maternal HDL (apoL, apoF, PON1, apoD, apoCs) have been described almost exclusively in the dense HDL3 fraction and relevant to its anti-oxidative function and role in innate immunity. Strikingly, PON1 mass and activity were 5-fold lower (p<0.01) in the fetus, which was accompanied by attenuation of anti-oxidant capacity of fetal HDL. Despite almost equal quantity of CETP in maternal and fetal HDL, its enzymatic activity was 55% lower (p<0.001) in the fetal circulation, whereas LCAT activity was not altered. These findings indicate that maternally derived HDL differs from fetal HDL with respect to its proteome, size and function. Absence of apoA-1, apoL and PON1 on fetal HDL is associated with decreased anti-oxidative properties together with deficiency in innate immunity collectively indicating distinct HDLs in fetuses.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoproteins - metabolism
- Apolipoproteins C - metabolism
- Apolipoproteins D - metabolism
- Apolipoproteins E - metabolism
- Aryldialkylphosphatase - metabolism
- Cholesterol Ester Transfer Proteins - metabolism
- Female -
- Fetus - metabolism
- Humans -
- Lipoproteins, HDL - chemistry Lipoproteins, HDL - metabolism
- Pregnancy -
- Find related publications in this database (Keywords)
- Fetal HDL
- PON1
- Antioxidative capacity
- Apolipoprotein