Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hochegger, K; Siebenhaar, F; Vielhauer, V; Heininger, D; Mayadas, TN; Mayer, G; Maurer, M; Rosenkranz, AR.
Role of mast cells in experimental anti-glomerular basement membrane glomerulonephritis.
Eur J Immunol. 2005; 35(10):3074-3082
Doi: 10.1002/eji.200526250
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Eller Kathrin
- Rosenkranz Alexander
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- Abstract:
- Recently, divergent reports on the role of mast cells (MC) in different glomerular diseases have brought our attention to their role in an accelerated model of anti-glomerular basement membrane (GBM) glomerulonephritis (GN). Genetically MC-deficient Kit(W)/Kit(W-v) mice, MC-reconstituted Kit(W)/Kit(W-v) mice and Kit+/+ control mice were subjected to anti-GBM GN. Kit(+/+) mice developed moderate proteinuria and glomerular damage following the induction of anti-GBM nephritis. In contrast, proteinuria and glomerular damage were dramatically increased in MC-deficient Kit(W)/Kit(W-v) mice. MC-reconstituted Kit(W)/Kit(W-v) mice showed proteinuria and glomerular damage comparable to Kit+/+ mice. A significant increase in infiltrating T cells and macrophages was detected in MC-deficient Kit(W)/Kit(W-v) mice as compared to Kit+/+ control mice and MC-reconstituted Kit(W)/Kit(W-v) mice. Accordingly, we observed an increase of TGF-beta1 mRNA in kidneys from Kit(W)/Kit(W-v) mice. Interestingly, we did not detect MC in the kidney using either Giemsa staining or RT-real-time PCR, but MC were found in the regional lymph nodes. Finally, mortality of Kit(W)/Kit(W-v) mice was significantly increased after the induction of anti-GBM GN due to uremia. Our report provides the first direct evidence that MC are protective in anti-GBM GN, possibly by modulating the influx of effector T cells and macrophages to inflammatory sites in the kidney.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Anti-Glomerular Basement Membrane Disease - immunology Anti-Glomerular Basement Membrane Disease - pathology
- CD4-Positive T-Lymphocytes - immunology
- Disease Models, Animal -
- Lymph Nodes - cytology Lymph Nodes - immunology
- Macrophages - immunology
- Mast Cells - immunology
- Mice -
- Mice, Mutant Strains -
- RNA, Messenger - analysis
- Reverse Transcriptase Polymerase Chain Reaction -
- Serine Endopeptidases - biosynthesis Serine Endopeptidases - immunology
- Transforming Growth Factor beta - immunology Transforming Growth Factor beta - metabolism
- Transforming Growth Factor beta1 -
- Tryptases -
- Find related publications in this database (Keywords)
- experimental glomerulonephritis
- mast cells
- inflammation