Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hochegger, K; Eller, P; Huber, JM; Bernhard, D; Mayer, G; Zlabinger, GJ; Rosenkranz, AR.
Expression of granzyme A in human polymorphonuclear neutrophils.
Immunology. 2007; 121(2):166-173
Doi: 10.1111/j.1365-2567.2006.02551.x
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- Führende Autor*innen der Med Uni Graz
- Eller Kathrin
- Rosenkranz Alexander
- Co-Autor*innen der Med Uni Graz
- Eller Philipp
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- Abstract:
- Polymorphonuclear neutrophils (PMNs) are the first line of defence against invading pathogens. They contain a multitude of antimicrobial and potentially cytotoxic substances packed in granules and secretory vesicles. Here, we show that granzyme A (GzmA) is constitutively expressed in human PMNs, but not in the promyelocytic cell line HL-60, by performing flow cytometry, western blot, enzyme-linked immunosorbent assay and quantitative polymerase chain reaction. To further track the intracellular localization of GzmA, we performed subcellular fractionation and found GzmA to be present in peroxidase-negative granules. Finally, stimulation with opsonized Escherichia coli or the bioincompatible haemodialysis membrane cuprophane led to up-regulation of GzmA expression at the transcriptional level as well as at the translational level. In conclusion, we provide clear evidence that GzmA is constitutively expressed in human PMNs and can be up-regulated upon stimulation. These findings may also indicate a role for GzmA in PMNs in defence against invading pathogens.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Fractionation -
- Cells, Cultured -
- Gene Expression Regulation, Enzymologic -
- Granzymes - blood Granzymes - genetics
- HL-60 Cells -
- Humans -
- Neutrophils - enzymology
- Phagocytosis -
- Reverse Transcriptase Polymerase Chain Reaction - methods
- Subcellular Fractions - enzymology
- Up-Regulation -
- Find related publications in this database (Keywords)
- neutrophils
- phagocytosis
- granzyme
- subcellular fractionation