Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Riederer, M; Ojala, PJ; Hrzenjak, A; Graier, WF; Malli, R; Tritscher, M; Hermansson, M; Watzer, B; Schweer, H; Desoye, G; Heinemann, A; Frank, S.
Acyl chain-dependent effect of lysophosphatidylcholine on endothelial prostacyclin production.
J Lipid Res. 2010; 51(10):2957-2966
Doi: 10.1194/jlr.M006536
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- Führende Autor*innen der Med Uni Graz
- Frank Sasa
- Riederer Monika
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Gastrager Michaela
- Graier Wolfgang
- Heinemann Akos
- Hrzenjak Andelko
- Malli Roland
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- Abstract:
- Previously we identified palmitoyl-lysophosphatidylcholine (16:0 LPC), linoleoyl-LPC (18:2 LPC), arachidonoyl-LPC (20:4 LPC), and oleoyl-LPC (18:1 LPC) as the most prominent LPC species generated by the action of endothelial lipase (EL) on high-density lipoprotein. In the present study, the impact of those LPC on prostacyclin (PGI(2)) production was examined in vitro in primary human aortic endothelial cells (HAEC) and in vivo in mice. Although 18:2 LPC was inactive, 16:0, 18:1, and 20:4 LPC induced PGI(2) production in HAEC by 1.4-, 3-, and 8.3-fold, respectively. LPC-elicited 6-keto PGF1α formation depended on both cyclooxygenase (COX)-1 and COX-2 and on the activity of cytosolic phospholipase type IVA (cPLA2). The LPC-induced, cPLA2-dependent (14)C-arachidonic acid (AA) release was increased 4.5-fold with 16:0, 2-fold with 18:1, and 2.7-fold with 20:4 LPC, respectively, and related to the ability of LPC to increase cytosolic Ca(2+) concentration. In vivo, LPC increased 6-keto PGF(1α) concentration in mouse plasma with a similar order of potency as found in HAEC. Our results indicate that the tested LPC species are capable of eliciting production of PGI(2), whereby the efficacy and the relative contribution of underlying mechanisms are strongly related to acyl-chain length and degree of saturation.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cells, Cultured -
- Cyclooxygenase 2 - genetics
- Endothelium, Vascular - metabolism
- Epoprostenol - biosynthesis
- Humans -
- Lysophosphatidylcholines - pharmacology
- Male -
- Mice -
- Mice, Inbred C57BL -
- RNA, Messenger - metabolism
- Find related publications in this database (Keywords)
- cyclooxygenase
- prostanoids
- calcium
- phospholipase A2
- endothelial cell
- arachidonic acid