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Prüller, F; Rosskopf, K; Mangge, H; Mahla, E; von Lewinski, D; Weiss, EC; Riegler, A; Enko, D.
Implementation of buffy-coat-derived pooled platelet concentrates for internal quality control of light transmission aggregometry: a proof of concept study.
J Thromb Haemost. 2017; 15(12):2443-2450 Doi: 10.1111/jth.13870 [OPEN ACCESS]
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Leading authors Med Uni Graz
Mangge Harald
Prüller Florian
Co-authors Med Uni Graz
Enko Dietmar
Mahla Elisabeth
Rosskopf Konrad
von Lewinski Dirk
Weiss Eva Christine

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Essentials In platelet function testing, standardized internal controls (IQC) are not commercially provided. Platelet function testing was performed daily on aliquoted pooled platelet concentrates. Pooled platelet concentrates showed stability for control purposes from Monday to Friday. Pooled platelet concentrates provide the necessary steadiness to serve as IQC material. Background Standardized commercially available control material for internal quality control (IQC) of light transmission aggregometry (LTA) is still lacking. Moreover, the availability of normal blood donors to provide fresh platelets is difficult in small laboratories, where 'volunteers' may be in short supply. Objectives To evaluate the implementation of buffy-coat-derived pooled platelet concentrates (PCs) for IQC material for LTA. Methods We used buffy-coat-derived pooled PCs from the blood bank as IQC material for LTA. On each weekend one PC was prepared (> 200 mL) and aliquoted from the original storage bag on a daily basis in four baby bags (40-50 mL), which were delivered from Monday to Friday to our laboratory. The IQC measurements of at least 85 work-weeks (from Monday to Friday) were evaluated with this new IQC material. LTA was performed on a four-channel Chronolog 700 Aggregometer (Chronolog Corporation, Havertown, PA, USA) (agonists: collagen, adenosine diphosphate [ADP], arachidonic acid [AA] and thrombin receptor activator peptide-6 [TRAP-6]). Results The medians of platelet aggregation from IQC measurements with collagen, ADP and AA from Monday to Friday were 68.0-59.5, 3.0-2.0 and 51.0-50.0%, respectively, and the mean of platelet aggregation with TRAP-6 was 71.2-66.4%. Conclusions Buffy-coat-derived pooled PCs serve as a reliable and robust IQC material for LTA measurements and would be beneficial for the whole laboratory procedure and employees' safety. © 2017 International Society on Thrombosis and Haemostasis.
Find related publications in this database (using NLM MeSH Indexing)
Adenosine Diphosphate - pharmacology
Arachidonic Acid - pharmacology
Collagen - pharmacology
Humans -
Oligopeptides - pharmacology
Platelet Aggregation - drug effects
Platelet Function Tests - methods
Platelet Function Tests - standards
Proof of Concept Study -
Quality Control -
Reference Standards -

Find related publications in this database (Keywords)
blood platelets
internal quality control
light transmission aggregometry
platelet function tests
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